中国药业2016，Vol.25Issue(1)：63-65,3.
烫骨碎补中总黄酮及柚皮苷的含量测定
Determination of the Content of Total Flavone and Naringin in Hot Rhizoma Drynariae
封传华 1李刚 1张静 1徐兰 1陶晓璇1
作者信息
- 1. 中国人民解放军第九四医院，江西 南昌 330002
- 折叠
摘要
Abstract
Objective To establish an HPLC method for the determination of total flavonoids and naringin in hot Rhizoma Drynariae. Methods UV assay wavelength was 283 nm, HPLC column was ODS2 C18 column ( 150 mm × 4. 6 mm, 5 μm ) , mobile phase was methanol-acetic acid-water ( 25 :4 :75 ) , the flow rate was 1 mL/min, the detection wavelength was 283 nm, the column temperature was 30 ℃, the injection volume was 10 μL. Results The absorption wavelength of total flavonoids in Drynaria maximum was 283 nm, and the mass concentration in the range of 0. 002 82-0. 112 8 g/L had a good linear relationship with absorbance;naringin concentra-tion in the range of 1. 128-112. 8 g/L had good linear relations with the peak area, and the recovery rate was 99. 38%, RSD was 1. 88% ( n=9 ) . Conclusion The method is simple, accurate, and reproducible and can be used in the content determination of total flavonoids and naringin in hot Rhizoma Drynariae.关键词
高效液相色谱法/烫骨碎补/总黄酮/柚皮苷Key words
HPLC/hot Rhizoma Drynariae/total flavonoids/naringin分类
医药卫生引用本文复制引用
封传华,李刚,张静,徐兰,陶晓璇..烫骨碎补中总黄酮及柚皮苷的含量测定[J].中国药业,2016,25(1):63-65,3.
