中国药业2016，Vol.25Issue(2)：70-71,72,3.
高效液相色谱法测定复方混悬滴眼液中苯扎氯铵含量
Determination of Benzalkonium Chloride in Loteprednol and Tobramycin Ophthalmic Suspension by HPLC
杨龙华 1宿洁 1陈涛 1卢京光1
作者信息
- 1. 山东省青岛市食品药品检验检测中心，山东 青岛 266071
- 折叠
摘要
Abstract
Objective To establish an HPLC method for the determination of benzalkonium chloride in Loteprednol and Tobramycin Ophthalmic Suspension. Methods After being filtrated,samples were separated by HPLC on a Waters Spherisorb S5 CN column(250 mm × 4. 6 mm,5 μm). The mobile phase was 0. 1% phosphoric acid water solution- acetonitrile- tetrahydrofuran(52 :39 :15). The detec-tion wavelength was 216 nm. The flowing speed was 1. 5 mL/min. The column temperature was 30 ℃. Results The mass concentration of benzalkonium chloride was in good linear relationship with the peak area in the range of 0. 04-0. 20 g/L ( r=0. 999 9,n=5), and the average recovery rate was 100. 22%,RSD=0. 59%( n=9). Conclusion The method is sensitive,accurate and convenient with good resolution,it can be applied to control the quality of Loteprednol and Tobramycin Ophthalmic Suspension.关键词
高效液相色谱法/苯扎氯铵/混悬滴眼液/防腐剂/含量测定Key words
HPLC/benzalkonium chloride/Loteprednol and Tobramycin Ophthalmic Suspension/antiseptic/content determination分类
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杨龙华,宿洁,陈涛,卢京光..高效液相色谱法测定复方混悬滴眼液中苯扎氯铵含量[J].中国药业,2016,25(2):70-71,72,3.
