中国中西医结合外科杂志2016，Vol.22Issue(2)：156-160,5.DOI:10.3969/j.issn.1007-6948.2016.02.013
螺旋藻藻蓝蛋白亚基的提取和免疫活性研究
Extract of Phycocyanin Subunits and Immunological Activity
赵小霞 1邱丽君 1宣成睿 2孙鹏 2周杨 3周玥 3杨丽敏2
作者信息
- 1. 天津市第四中心医院检验科 天津 300140
- 2. 内蒙古医科大学病原生物研究中心 呼和浩特 010110
- 3. 内蒙古燚阳生物科技有限公司 呼和浩特010059
- 折叠
摘要
Abstract
Objective To explore the crude extract technology for phycocyanin subunits from spirulina and the immunological activity. Methods Spirulina powder were wall-broken at low temperature, and phycocya?nin was extracted by thick fibrous membrane from the centrifugal supernatant, then purified by SephadexG-50 gel chromatography. The purity and molecular weight were tested by SDS-PAGE, the absorbance at the wave?length of 620 nm and 280 nm were detected by infrared spectroscopy and UV/ visible spectrophotometer scan?ning spectrum. And then the effect of phycocyanin subunits extract on the mouse spleen lymphocytes was observed. Results The extracts of phycocya?nin subunits , No. 1 and No. 2 were dark green and blue dry powder respectively. They were water-solu?ble,and storage-stable at room temperature storage. In SDS-PAGE, both the extracts had a protein bands with molecular weights of 17kDa. In the infrared spectro?gram, spirulina powder showed 1 absorption peak at 1656 cm-1 wavelength, No. 1 extract showed 3 absorption peaks at 1655 cm-1, 1633 cm-1, and 1594 cm-1, No. 2 extract showed 2 absorption peaks at 1631 cm-1 and 1602 cm-1, and in the residue had 1 absorption peaks at 1658 cm-1. In UV-vis,the absorption peaks of No. 1 extract were at 263.5nm、619.5 nm wavelength, and of No. 2 extract, were at the 263.5 nm and 619.5 nm. The puritys of No. 1 extract and No. 2 extract (A620/A280) waere 0.78 and 0.85, and the recoverys of No. 1 extract and No. 2 extract were 15% and 20% respectively. Phycocyanin subunit extracts promoted the proliferation of the mouse spleen lymphocytes. Conclusion The crude extracts of phycocyanin subunits from spirulina by low tempera?ture have immunological activity.关键词
低温提取/藻蓝蛋白亚基/免疫活性Key words
Low temperature extract/phycocyanin subunit extract/immunological activity分类
医药卫生引用本文复制引用
赵小霞,邱丽君,宣成睿,孙鹏,周杨,周玥,杨丽敏..螺旋藻藻蓝蛋白亚基的提取和免疫活性研究[J].中国中西医结合外科杂志,2016,22(2):156-160,5.
