针灸临床杂志2016，Vol.32Issue(1)：64-67,4.
头针电刺激对急性脑梗死大鼠 Bax与 Bcl－2蛋白的影响
Effect of Scalp Acupuncture and Electrical Stimulation on Protein Bax and Bcl-2 in Rats with Acute Cerebral Infarction
摘要
Abstract
Objective:To observe the changes of protein Bax and Bcl -2 after treating acute cerebral infarction of rats with scalp acupuncture and electrical stimulation , and to discuss the mechanism on how these treatments affect cerebral infarction .Methods:Divide healthy female wistar rats into a treatment group , a model control group and a sham operation group randomly .Then divide every group into seven -days group and fourteen -days group by their treatment starting time after infarction ,with fifteen rats every time point .Make focal cerebral infarction models by suture method , then from the first day after making models , intervene the treatment group with scalp acupuncture , choose BaiHui and DaZhui acupoints , stimulate the acupoints with 100 Hz electrical wave , once per day , 30 minutes every time .For other two groups of rats , just fix them on the table with no in-tervention .After seven days and fourteen days , when the treatment was over , cut off their heads and take out the brains .Examine the infarction brain tissues with Western blot technology and determine the expressions of protein Bax and Bcl -2.Results:The expression of protein Bax was descended gradually , but the expression of Bcl-2 was ascended in the treatment group;in the model control group , the expressions of two proteins were descended .Compared with the model control group , the expressions of protein Bax in the treatment group on the seventh day and fourteenth day were descended ,and the expression of Bcl -2 was ascended .The difference had a statistical significance (P<0.05).Conclusion:Scalp acupuncture with electrical stimulation can effec-tively protect the infarction brain tissues .It can descend the expression of protein Bax , ascend the expression of Bcl-2 at the same time , maximize the difference between the expression of the two proteins , inhibit apoptosis in the ischemic regions .This might be the mechanism on how scalp acupuncture with electrical stimulation im-prove the infarction rats .关键词
脑梗死/头针/电刺激/Bax/Bcl-2Key words
Cerebral infarction/Scalp acupuncture/Electrical stimulation/Bax/Bcl-2分类
医药卫生引用本文复制引用
李晓宁,綦雪巍,王振宇..头针电刺激对急性脑梗死大鼠 Bax与 Bcl－2蛋白的影响[J].针灸临床杂志,2016,32(1):64-67,4.基金项目
黑龙江省自然科学基金面上项目，编号H201466。 （）
