浙江农林大学学报2016，Vol.33Issue(2)：183-190,8.DOI:10.11833/j.issn.2095-0756.2016.02.001
雷竹和拟南芥SOC1多聚体差异性分析
Oligomeric status of the SOC1 gene from Phyllostachys violascens and Arabidopsis thaliana
摘要
Abstract
MADS-box genes are a super family, which can form different polymers to mediate the development of floral primordia and floral organs. The important one of MADs-box genes, SOC1 [suppressor of overexpres-sion of cytochrome oxidase 1], determine the flowering time. The MADS-box transcription factor, SOC1 con-tains four different functions domains: MADS, I, K, and C. The K domain controls the homologous or heterolo-gous protein polymer formation. The I domain could stabilize the MADS-box proteins binding the DNA. In or-der to control the floral development, MADS-box proteins can form different complex. Visible difference in flo-ral timing is demonstrated in Phyllostachys violascens and Arabidopsis thaliana. A. thaliana is certain in floral timing while Phyllostachys violascens is not. Though we cannot compare the difference in floral timing with the difference in SOC1 complex formation, it is still interesting. MADS-box genes such as SOC1 containing four different functions: MADS, I, K and C, control floral timing. To determine whether SOC1 ’s from different species demonstrated similar patterns in forming a complex, this study analyzed the differences of polymer for-mation for SOC1 that originated from Phyllostachys violascens and Arabidopsis thaliana using a yeast two-hy-brid assay. Results of the assay showed that the full-length AtSOC1 gene from A. thaliana formed strong homo-polymers with the I and K domains being the main regions for formation of the interaction. However, the full-length SOC1 from Phyllostachys violascens (PvSOC1) did not form a homodimer. Thus, even though the K pro-tein domain could form a homodimer, the presence of the I protein domain disrupted the dimer meaning the I domain could be a key factor in SOC1 polymer formation; the role of the I domain in floral timing should be verified with a plant transgene study.关键词
植物学/MADS-box/SOC1/雷竹/拟南芥/酵母双杂交/多聚体状态/I和 K结构域Key words
botany/MADS-box/SOC1/Phyllostachys violascens/Arabidopsis thaliana/yeast two-hybrid/poly-mer/I and K protein domain分类
农业科技引用本文复制引用
施泉,陈晓沛,林新春,徐永汉,徐英武..雷竹和拟南芥SOC1多聚体差异性分析[J].浙江农林大学学报,2016,33(2):183-190,8.基金项目
国家自然科学基金资助项目（31270715，31000295）；浙江省林学一级重中之重学科开放基金资助项目（KF201304）；国家重点基础研究发展计划（“973”计划）项目（2012CB723008）；浙江农林大学研究生创新基金资助项目 （）
