肿瘤药学2016，Vol.6Issue(1)：35-38,4.DOI:10.3969/j.issn.2095-1264.2016.01.08
Pim-1激酶对小鼠CD4+T细胞分化的影响
Effects of Pim-1 Kinase on the Differentiation of Naïve CD4+T Cells
摘要
Abstract
Objective The aim of this study was to understand more about the effects of Pim-1 kinase on the differentiation of naïve CD4+T. Methods After PIM-Inh block, the naïve CD4+T cells were induced by different cytokines. The expression of T cell master transcription factors (T-bet, ROR-γt, GATA-3, Foxp3) were measured by Western Blot. And the production of lymphocytes cytokines (IFN-γ, IL-4, TGF-β, IL-17) was determined by ELSIA. Results The expressions of T-bet, GATA-3, Foxp3 and ROR-γt pro-teins in differentiation groups of naïve CD4+T cells were significantly higher than in the controls, and so were the expressions of IL-4, TGF-β, IFN-γand IL-17 cytokines. Administration of PIM-Inh resulted in inhibition of T-bet and ROR-γt expression in a dose-dependent manner. PIM-Inh also inhibited the production of IFN-γand IL-17 in a dose-dependent manner. Conclusion These data suggested that Pim-1 may play an important role in the differentiation of naïve CD4+T cells into Th1 and Th17 cells.关键词
Pim-1/CD4+T细胞Key words
Pim-1/CD4+Tcell分类
医药卫生引用本文复制引用
申月明,赵艳,陈伟,王俊,谭丽,曾亚..Pim-1激酶对小鼠CD4+T细胞分化的影响[J].肿瘤药学,2016,6(1):35-38,4.基金项目
国家自然科学基金（81200277）；湖南省医药卫生科研计划基金资助项目（No.B2012-133）。 （）
