肿瘤药学2016，Vol.6Issue(1)：39-43,5.DOI:10.3969/j.issn.2095-1264.2016.01.09
胡桃醌衍生物MAM抑制人乳腺癌MDA-MB-231细胞增殖的体外研究
Effects of 2-Methoxy-6-acetyl-7-methyljuglone on Proliferation of Human Breast Cancer MDA-MB-231 Cells invitro
林思思 1曾行 2罗丹 1陈彤丹1
作者信息
- 1. 浙江省人民医院，浙江 杭州，310014
- 2. 浙江大学附属第一医院，浙江 杭州，310003
- 折叠
摘要
Abstract
Objective To investigate the effects of 2-Methoxy-6-acetyl-7-methyljuglone (MAM) extracted from Polygonum cuspidatum (Hu Zhang) on the proliferation of human breast cancer MDA-MB-231 cells and its possible mechanisms. Method Human normal liver cellLO2andhumanbreastcancercells(MDA-MB-231andMDA-MB-468)wereculturedin vitro.TheeffectsofMAMonthegrowthin-hibition ratio of cells were evaluated by the Cell Counting Kit-8 (CCK8). The effects of MAM on the apoptosis of MDA-MB-231 cells were detected through morphological observation by Hoechst33258 staining. The effects of MAM on the cell cycle of MDA-MB-231 cells were detected through flow cytometric analysis (FCM). The expressions of related proteins were analyzed by western blot. Results MAM signifi-cantly inhibited the growth and proliferation of MDA-MB-231 cells in a dose-and time-dependent manner. Typical morphological change of apoptosis occurred in MDA-MB-231 cells when treated with MAM for 24 h, and increased cells were arrested in G2/M phase. Western blot showed the expressions of Cdk1 and CyclinB1 were depressed, and protein expression of p-Cdk1 (Thr14) was up-regulated (P﹤0.05). Conclusion MAM inhibits the proliferation of MDA-MB-231 cells through G2/M cell cycle arrest induction, which is mediated by depres-sion of Cdk1-CyclinB1 complex.关键词
2-甲氧基-6-乙酰基-7-甲基胡桃醌/乳腺癌/增殖/Cyclin B1/Cdk1Key words
2-Methoxy-6-acetyl-7-methyljuglone/Breast cancer/Proliferation/Cyclin B1/Cdk1分类
医药卫生引用本文复制引用
林思思,曾行,罗丹,陈彤丹..胡桃醌衍生物MAM抑制人乳腺癌MDA-MB-231细胞增殖的体外研究[J].肿瘤药学,2016,6(1):39-43,5.
