肿瘤药学2016，Vol.6Issue(1)：49-53,5.DOI:10.3969/j.issn.2095-1264.2016.01.11
普伐他汀对人胰腺癌细胞SW1990增殖和凋亡的影响及其机制研究
Effects of Pravastatin on the Proliferation and Apoptosis of Human Panceatic Cancer Cell SW1990 and Its Mechanisms
张敏 1孔雁 2姜达 2程敏菊 1郭治1
作者信息
- 1. 邢台市人民医院肿瘤内科，河北 邢台，054001
- 2. 河北医科大学第四医院肿瘤内科，河北 石家庄，050000
- 折叠
摘要
Abstract
Objective To investigate the effects of pravastatin on the proliferation and apoptosis of human pancreatic cancer cells SW1990 and its possible mechanisms. Methods SW1990 cells were cultured in vitro and treated with different concentrations (0, 5, 10, 20μmol·L-1) of pravastatin. Effects of pravastatin on the proliferation of SW1990 cells were measured by MTT colorimetric method. The apoptosis of SW1990 cells and expression of p21Ras, Bcl-2 and Bax were examined with flow cytometry. The analysis of mRNA of Bcl-2 and Bax was shown by RT-PCR. Results After SW1990 cells were treated with pravastatin, the cell proliferation was inhib-ited and the cell apoptosis was increased. The protein expression of p21Ras and Bcl-2 decreased while the protein expression of Bax increased. The mRNA level of Bcl-2 decreased but that of Bax increased. Conclusions Pravastatin could inhibit the proliferation of SW1990 cell and induced its apoptosis. Its mechanism may involve the down-regulation of p21Ras, Bcl-2 and up-regulation of Bax.关键词
普伐他汀/胰腺癌/增殖/凋亡/SW1990Key words
Pravastatin/Panceaticcancer/Proliferation/Apoptosis/SW1990分类
医药卫生引用本文复制引用
张敏,孔雁,姜达,程敏菊,郭治..普伐他汀对人胰腺癌细胞SW1990增殖和凋亡的影响及其机制研究[J].肿瘤药学,2016,6(1):49-53,5.
