作物学报2016，Vol.42Issue(5)：627-632,6.DOI:10.3724/SP.J.1006.2016.00627
ω-黑麦碱基因沉默对小麦1B/1R易位系加工品质的影响
Effect of ω-Secalin Gene Silencing on Processing Quality of Wheat 1B/1R Translocation Line
摘要
Abstract
Omega-secalin is an important factor for the poor processing quality of wheat 1B/1R translocation lines. In this study, an expression vector of silencing ω-secalin gene was constructed and transferred into wheat variety Jinhe 9123 via Agrobacterium tumefaciens strain EH105. The three T0 transgenic plants obtained were consecutively multiplied after PCR validation until the T4 lines. Acid-PAGE assay indicated that total amount of ω-secalins in the transgenic lines decreased 53% on average compared with that in their respective control. In the three homozygous transgenic lines, gluten index, precipitation value, and stabilization time increased significantly, whereas the agronomic traits, such as plant height, grain number per spike, 1000-grain weight and plot yield, had no significant changes compared with those in the wild type. These results suggest that silencing ω-secalin genes can improve the processing quality of wheat 1B/1R translocation lines on the premise of not affecting grain yield.关键词
小麦1B/1R易位系/黑麦碱/RNA干扰/基因沉默/加工品质Key words
Wheat 1B/1R translocation line/Secalin/RNAi/Gene silencing/Processing quality引用本文复制引用
柴建芳,王海波,马秀英,张翠绵,董福双..ω-黑麦碱基因沉默对小麦1B/1R易位系加工品质的影响[J].作物学报,2016,42(5):627-632,6.基金项目
本项目由河北省自然科学基金项目(C2014301005)资助. This study was supported by the Natural Science Foundation of Hebei Province, China (C2014301005). （C2014301005）
