作物学报2016，Vol.42Issue(5)：633-640,8.DOI:10.3724/SP.J.1006.2016.00633
高分子量谷蛋白单亚基缺失对软质小麦宁麦9号加工品质的影响
Effect of HMW-GS Deletion on Processing Quality of Soft Wheat Ningmai 9
摘要
Abstract
Gene knockout is an effective approach to investigate gene function of high-molecular-weight glutenin subunit (HMW-GS). In this study, we developed a set of single HMW-GS deletion lines of Ningmai 9 (soft wheat) to understand the effects of HMW-GS deletion on kernel quality, quantity of gluten protein fractions and processing quality. Among the 29 lines tested, three lines were wild type and the remaining were knockout mutants including Glu-A1x, Glu-B1x,Glu-B1y, Glu-D1x, and Glu-D1y deletion types of five, seven, five, five, and four lines, respectively. Compared with the wild type, the HMW-GS deletion lines had no significant difference in flour protein content, wet gluten content, kernel hardness, and solvent retention capacity. All the single HMW-GS deletion types decreased the ratios of glutenin-to-gliadin and HMW-GS-to-LMW-GS in quantity, and the decreases in Glu-B1x and Glu-D1x deletion types were significant atP< 0.05. In the wild type, mixograph peak time was 2.00 min and TIMEX width was 4.57%; whereas, those in the HMW-GS deletion lines were significantly lower (P < 0.05), varying from 1.38 min to 1.64 min and from 3.38% to 3.98%, respectively. Particularly, the Glu-B1x and Glu-D1x deletion lines showed the lowest mixograph peak time and TIMEX width. Although similar sugar snap cookie diameter was observed among the five deletion types, Glu-B1x, Glu-B1y, and Glu-D1y deletion types showed significantly higher cookie diameter than the wild type (P < 0.05). In this study, single deletion of HMW-GS weakened gluten strength and improved sugar snap cookie processing quality, indicating that HMW-GS knockout can be used to improve soft wheat quality.关键词
HMW-GS缺失/宁麦9号/加工品质Key words
HMW-GS deletion/Ningmai 9/Processing quality引用本文复制引用
张平平,马鸿翔,姚金保,周淼平,张鹏..高分子量谷蛋白单亚基缺失对软质小麦宁麦9号加工品质的影响[J].作物学报,2016,42(5):633-640,8.基金项目
本研究由国家自然科学基金项目(31101146), 江苏省农业科技自主创新资金项目(CX(14)2002)和国家现代农业产业技术体系建设专项(CARS-03)资助. This study was supported by the National Natural Science Foundation of China (31101146), the Indigenous Innovation Foundation of Jiangsu Provincial Agricultural Science and Technology of China (CX(14)2002), and the China Agriculture Research System (CARS-03). （31101146）
