作物学报2016，Vol.42Issue(5)：667-674,8.DOI:10.3724/SP.J.1006.2016.00667
水稻叶片早衰突变体ospls3的生理特征和基因定位
Physiological Characteristics and Gene Mapping of a Precocious Leaf Senes-cence Mutant ospls3 in Rice
摘要
Abstract
Leaf senescence is the final stage of leaf development. However, premature aging of functional leaves leads to yield reduction and quality decline. Thus, it is very important for developing novel crop germplasms with delayed leaf-senescence characteristics through investigating the molecular mechanism of leaf senescence. In this study, anospls3(Oryza sativa preco-cious leaf senescence 3) mutant, produced by 60Coγ-radiation treatment ofindica cultivar N142, was identified. The symptoms of the premature senescence mutant presented firstly at tillering stage showing brown leaf tip and brown spots in top part of leaf blade, then spread rapidly to basal part of leaf blade and led leaf to die. The physiological analysis indicated that, in theospls3 mutant, the content of chlorophyll was the highest in the flag leaf, the following was in second-top and third-top leaves, but all of them were significantly lower than those in the wild type. The contents of MDA, O2-, and H2O2 and the activities of SOD and POD among the top three leaves in the wild type maintained similar levels, which were significantly lower than those in the mu-tant. The soluble protein contents and the activity of CAT had no significant difference among top three leaves in the wild type while significantly decreased in the mutant. Genetic analysis verified that theospls3 is a recessive mutant and was mapped in a 294 kb interval between RM6953 and RM28753 on the long arm of chromosome 12, which establishes a solid foundation for further cloning and functional studies of this gene.关键词
水稻/ospls3/叶片早衰/生理分析/基因定位Key words
Rice/ospls3/Precocious leaf senescence/Physiological analysis/Gene mapping引用本文复制引用
龚盼,黎坤瑜,黄福灯,韦荔全,杨茜,程方民,潘刚..水稻叶片早衰突变体ospls3的生理特征和基因定位[J].作物学报,2016,42(5):667-674,8.基金项目
本研究由国家自然科学基金项目(31271691)和国家转基因生物新品种培育重大专项(2013ZX08001-002)资助. This study was supported by the National Natural Science Foundation of China (31271691) and the Major Project of China on New Varieties of GMO Cultivation (2013ZX08001-002). （31271691）
