作物学报2016，Vol.42Issue(5)：696-705,10.DOI:10.3724/SP.J.1006.2016.00696
基于蛋白质组学的高丹草苗期杂种优势分析
Analysis of Heterosis in Sorghum-Sudangrass Hybrid Seedlings Based on Pro-teomics
摘要
Abstract
Sorghum-sudangrass hybrids are typically used for studying heterosis in forage crops. In this study, we carried out pro-teomic research on sorghum-sudangrass hybrids and their parents at the three-leaf stage by two dimensional electrophoresis-based proteomics and bioinformatic methods. More than 400 protein spots were detected, in which 34 proteins showed significant dif-ferences between hybrid and parents in expression, including dominant expression (showing three single-parent silent, seventeen high-parent and five low-parent expression) and overdominant expression (showing one hybrid-specific, six above-high-parent, two below-low-parent expression). Thus, we speculated that dominant and overdominant effects play key roles, and dominant effect is a major factor in the formation of heterosis in sorghum-sudangrass hybrid. Moreover, 27 out of 34 proteins were related to eight functional categories, i.e., photosynthesis, carbohydrate metabolism, stress response, ATP synthesis, protein synthesis, electron transfer, signal transduction and unknown. The up-regulated photosynthetic proteins were the biggest category, which indicates that photosynthesis in the leaves of sorghum-sudangrass hybrid is enhanced resultig in producing more organic matter, so that showing heterosis. The identified key node proteins in the interaction networks were the potential target proteins for future genetic manipulation of the specific proteins of heterosis. Our findings provide a theoretical basis on heterosis analysis of sor-ghum-sudangrass hybrids, which is potentially useful for other forage plants.关键词
高丹草/叶片/杂种优势/蛋白质组Key words
Sorghum-sudangrass hybrid/Leaves/Heterosis/Proteomics引用本文复制引用
韩平安,逯晓萍,米福贵,张瑞霞,李美娜,薛春雷,董婧,丛梦露..基于蛋白质组学的高丹草苗期杂种优势分析[J].作物学报,2016,42(5):696-705,10.基金项目
本研究由国家自然科学基金项目(31160302, 31460375)和呼和浩特市科技计划项目(2012-重计-8-2)资助. This study was supported by the National Natural Science Foundation of China (31160302, 31460375) and the Science and Technology Plan Projects of Hohhot (2012-major plans-8-2). （31160302, 31460375）
