作物学报2016，Vol.42Issue(5)：706-713,8.DOI:10.3724/SP.J.1006.2016.00706
普通小麦类胡萝卜素组分的超高效液相色谱分离方法
Establishment of Ultra Performance Liquid Chromatography (UPLC) Protocol for Analyzing Carotenoids in Common Wheat
摘要
Abstract
Carotenoids is an important criterion in the assessment of color and nutritional qualities of end-use products in common wheat. In this study, grain powders of Zhongmai 175 (soft wheat) and Zhongyou 206 (hard wheat) were used as samples to de-velop an optimal procedure for extraction and separation of carotenoids compositions extracted from flours using ultra perform-ance liquid chromatography (UPLC). The most effective extraction of carotenoids extraction was obtained using the solvent sys-tem of N-hexane; acetone of 80:20 (v/v, 0.1% BHT w/v) under the oscillation condition of 300 r min-1, 35oC, and 1 h. The separa-tion was conducted using YMC C30 Carotenoid column (100.0 mm × 4.6 mm, 3 μm) with photodiode array (PDA) detector, and the column was thermostated at 35°C. Under a gradient system consisting of acetonitrile:methanol:water:triethylamine (81:14:5:0.05, v/v/v/v) (A) and methanol: ethylacetate:triethylamine (68:32:0.05, v/v/v) (B) at a constant flow rate of 0.4 mL min-1, the carotenoids of common wheat flour samples can be well separated in less than 25 min. Carotenoids were detected at 450 nm. All the present results provided useful information for carotenoids compositions and quality improvement.关键词
普通小麦/类胡萝卜素组分/超高效液相色谱Key words
Triticum aestivum L./Carotenodis compositions/UPLC引用本文复制引用
李文爽,夏先春,何中虎..普通小麦类胡萝卜素组分的超高效液相色谱分离方法[J].作物学报,2016,42(5):706-713,8.基金项目
本研究由引进国际先进农业科学技术计划(948计划)项目[2011-G(3)4]和科技部小麦玉米高效分子标记体系的研发和应用项目(2013DFG30530)资助. This study was supported by the Program of Introducing International Super Agricultural Science and Technology [2011-G(3)4] and Ministry of Science and Technology (2013DFG30530). （948计划）
