作物学报2016，Vol.42Issue(5)：714-724,11.DOI:10.3724/SP.J.1006.2016.00714
可溶性寡糖和小分子的热激蛋白与杂交水稻种子成熟过程中发芽能力及种子活力相关
Soluble Oligosaccharide and Small Heat Shock Protein Correlated with Seed Germination and Vigor during Hybrid Rice Seed Maturation
摘要
Abstract
To study the relationship between seed soluble oligosaccharide, small heat shock protein (sHSPs) and seed vigor, we systematically studied their changes during hybrid rice seed maturity. The mean fuzzy subordinate function values of germination percentage, germination energy, germination index, vigor index and mean germination time, root number, root length, shoot length and seedling weight were analyzed. The results indicated that seeds harvesting at 19 to 34 days after pollination (DAP) had a higher viability and vigor. The seed moisture content had a decline trend, with a decrease from 48.2% at 10 DAP to 23.9% at 22 DAP. The raffinose content increased significantly, the fructose content kept decreasing, the contents of stachyose and glucose significantly increased firstly and then decreased during seed development. The relative expression levels of 64SHsp18.0 and Os03g0267200 genes increased significantly from 10 to 19 DAP, and then decreased. The moisture content, the contents of raffi-nose, stachyose and sucrose, thousand seed weight of fresh seeds and dry seeds, relative transcriptions of 64SHsp18.0 and Os03g0267200 genes were all significantly correlated with seed germination during seed maturity.关键词
杂交水稻/种子/成熟/小热激蛋白/寡糖/活力Key words
Hybrid rice/Seed/Maturity/sHSPs/Oligosaccharide/Vigor引用本文复制引用
朱丽伟,曹栋栋,付玉营,胡琦娟,利站,关亚静,胡伟民,胡晋..可溶性寡糖和小分子的热激蛋白与杂交水稻种子成熟过程中发芽能力及种子活力相关[J].作物学报,2016,42(5):714-724,11.基金项目
本研究由国家公益性行业(农业)科研专项(201203052),浙江省科技厅项目(2013C02005, 2013C32023),浙江省自然科学基金项目(LZ14C130002, LY15C130002)和江苏省现代作物生产协同创新中心项目资助. This study was supported by the China Special Fund for Agro-Scientific Research in the Public Interest (201203052), the Subject Fund of Zhejiang Province Science and Technology Hall (2013C02005, 2013C32023), the Natural Sciences Fund of Zhejiang Province (LZ14C130002, LY15C130002), and Jiangsu Collaborative Innovation Center for Modern Crop Production. （农业）
