作物学报2016，Vol.42Issue(5)：758-767,10.DOI:10.3724/SP.J.1006.2016.00758
玉米抗灰斑病QTL元分析及其验证
Meta-analysis and Validation of QTL for Resistance to Gray Leaf Spot in Maize
摘要
Abstract
Gray leaf spot (GLS) is one of the most severe leaf diseases of maize worldwide. The breeding progress for resistance to GLS has been seriously hindered by less knowledge about QTL number, QTL intervals and mechanism of GLS. Based on meta-analysis, we conformed 13 consensus QTL regions from 14 articles on resistance to gray leaf spot of maize. One backcross with inbred line 81162 as recurrent parent and inbred line CN165 as donor parent, was used to test the consensus of those 13 QTL regions on the basic of linkage disequilibrium getting more than 20 partial separated loci. High level of partial separation indicated that there were QTLs with high effects of resistance to GLS on chromosome 1 and chromosome 4. On chromosome 1, the donor genes closed to markers of umc2227, bnlg1832, umc1243, umc2025, umc1515, umc1297, and umc1461 showed the frequency over 50%. Therefore, we inferred that there were a few of highly linked QTLs on chromosome 1. The resistant QTL on chromo-some 4 was located between markers bnlg2291 and umc1194. Consequently, this study could lay a foundation for the QTL fine mapping on chromosome 1 and chromosome 4 in the donor parent CN165.关键词
灰斑病/元分析/一致性QTL/回交导入系/主效QTLKey words
Gray leaf spot/Meta-analysis/Consensus QTLs/Backcross introgression lines/Major QTL引用本文复制引用
闫伟,李元,宋茂兴,张旷野,孙铭泽,瞿会,李凤海,钟雪梅,朱敏..玉米抗灰斑病QTL元分析及其验证[J].作物学报,2016,42(5):758-767,10.基金项目
本研究由国家自然科学基金项目(31301322)和辽宁省科技特派项目(2014215031)资助. This study was supported by the National Natural Science Foundation of China (31301322) and the Special Talent Appointment in Techno-logy of Liaoning Province (2014215031). （31301322）
