中国现代中药2016，Vol.18Issue(3)：307-311,5.DOI:10.13313/j.issn.1673-4890.2016.3.011
加味小柴胡汤抗幽门螺杆菌的有效物质部位群研究
Study on Anti-Helicobacter pylori Effective Fractions of Modified Xiao Chaihu Decoction
胡丽娟 1刘蔚 1吴欢欢 1李力 2周爱军 3刘焱文1
作者信息
- 1. 湖北中医药大学,湖北 武汉 430065
- 2. 武汉市中医医院,湖北 武汉 430050
- 3. 东莞市中医院,广东 东莞 523000
- 折叠
摘要
Abstract
Objective:To investigate the anti-helicobacter pylori effect of the Modified Xiao Chaihu Decoction and their isolated components in vitro.Methods:The means of systematic solvent extraction was used to separate volatile oils, alkaloids,phenolic acids,saponins and polysaccharides from the decoction.The inhibition zones of the decoction and their isolated components were surveyed through agar diffusion method,and the minimal inhibitory concentrations(MICs)were also determined by using fluid dilution method.Results:The bacterial inhibition diameter and MIC of the decoction were(6.90 ± 0.63)mm and 0.125 g·mL -1 of the crude drug,the bacterial inhibition diameter and MIC of the volatile oils were (7.46 ±0.47)mm and 0.500 g·mL -1 of the crude drug,the bacterial inhibition diameter and MIC of the alkaloids were (7.28 ±0.21 )mm and 0.130 g·mL -1 of the crude drug,the bacterial inhibition diameter and MIC of the phenolic acids were(7.46 ±0.10)mm and 0.506 g·mL -1 of the crude drug,the bacterial inhibition diameter and MIC of the saponins were(7.29 ±0.10)mm and 0.246 g·mL -1 of the crude drug,the bacterial inhibition diameters of the polysaccharides was zero.Conclusion:The volatile oils,alkaloids,phenolic acids and saponins of the Modified Xiao Chaihu Decoction are anti-helicobacter pylor active fractions.关键词
加味小柴胡汤/幽门螺杆菌/有效物质部位群/抑菌圈/MICKey words
Modified Xiao Chaihu Decoction/Helicobacter pylori/effective fractions/bacterial inhibition diameter/MIC引用本文复制引用
胡丽娟,刘蔚,吴欢欢,李力,周爱军,刘焱文..加味小柴胡汤抗幽门螺杆菌的有效物质部位群研究[J].中国现代中药,2016,18(3):307-311,5.
