中国现代中药2016，Vol.18Issue(3)：329-334,6.DOI:10.13313/j.issn.1673-4890.2016.3.017
贡菊不定芽离体诱导增殖培养条件的优化
Optimization of Culture Conditions in vitro of Adventitious Buds Induction and Proliferation of Dendranthema morifolium Tzvel.cv.Gongju
摘要
Abstract
Objective:The culture conditions of adventitious buds induction and proliferation were optimized to lay a foundation for establishing and perfecting the rapid propagation system of Dendranthema morifolium.Methods:Single factor test,combination test and orthogonal test were used,also the compositions of basic medium were optimized to investigate the effect of different plant growth regulator on adventitious buds induction and proliferation.Results:The results showed that the optimum cytokinin for induction was 6-BA;the best medium for induction was MS +6-BA2.0 mg·L -1 +NAA0.1 mg·L -1 or 6-BA2.0 mg·L -1 +IBA0.1 mg·L -1 ;the best medium for propagation was MS +6-BA2.0 mg·L -1 +NAA0.02 mg·L -1 +IBA0.1 mg·L -1 ;the optimized basic medium compositions were:basic MS macronutrients concentration +1.5 times Fe +sucrose 20 g·L -1 +coconut water 30 ml·L -1 .Conclusion:6-BA has obvious effect,and also IBA and NAA had regulating effect on induction and propagation of adventitious beds,in addition,the improved medium could promote adventitious buds growth.关键词
贡菊/不定芽诱导与增殖/条件优化Key words
Dendranthema morifolium/Adventitious buds induction and proliferation/optimization引用本文复制引用
张家瑛,潘超美,苏家贤,肖辛垣..贡菊不定芽离体诱导增殖培养条件的优化[J].中国现代中药,2016,18(3):329-334,6.基金项目
2014公益性行业科研专项(201407002) （201407002）
