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不同种质黄芩中黄酮类成分测定及分析

高晓霞 徐荣 陈君 于晶 杨成民 刘赛 张永清 彭艳丽

中国现代中药2016,Vol.18Issue(3):343-347,352,6.
中国现代中药2016,Vol.18Issue(3):343-347,352,6.DOI:10.13313/j.issn.1673-4890.2016.3.020

不同种质黄芩中黄酮类成分测定及分析

Determination and Analysis of Flavonoids Ingredients of Different Scutellariae Radix Germplasms

高晓霞 1徐荣 2陈君 2于晶 2杨成民 2刘赛 2张永清 2彭艳丽1

作者信息

  • 1. 山东中医药大学,山东 济南 250355
  • 2. 中国医学科学院 药用植物研究所,北京 100193
  • 折叠

摘要

Abstract

Objective:To provide a basis for selecting high content of flavonoids ingredients of Scutellariae Radix germplasms by determination and analysis on three flavonoids ingredients and the total flavonoids of different Scutellariae Radix germplasms.Methods:The content of baicalin,baicalein and wogonin were determined by HPLC.And the total content of flavonoids of different Scutellariae Radixgermplasm was determined by ultraviolet-visible spectrophotometry.Principal component analysis and grey pattern recognition method was applied to comprehensive evaluation of different Scutellariae Radix germplasms quality.Results:Variance analysis results showed that the content of baicalin,wogonin and the total flavonoids were extremely significantly different among different germplasms.The baicalin and total flavonoids in germplasm 13 was higher than other germplasms.Germplasm 1 and 7,of which baicalin,baicalein and total flavonoids were all high.The analysis results of grey pattern recognition method verified that the Scutellariae Radix of germplasm 1,7 and 13 are optimal germplasms,which can be as selection objects of Scutellaria baicalensis new varieties.Conclusion:The improved HPLC method is fit for determination for flavonoids ingredients of scutellariae Radix.There are significant difference between different Scutellariae Radix germplasms.Principal component analysis and the grey pattern recognition method is suitable for quality evaluation of different Scutellariae Radix germplasms.

关键词

黄芩/黄酮类成分/主成分分析/灰色模式识别法

Key words

Scutellariae Radix/flavonoids ingredients/principal component analysis/grey pattern recognition method

引用本文复制引用

高晓霞,徐荣,陈君,于晶,杨成民,刘赛,张永清,彭艳丽..不同种质黄芩中黄酮类成分测定及分析[J].中国现代中药,2016,18(3):343-347,352,6.

基金项目

中医药行业科研专项(201107011) (201107011)

工信部中药材扶持资金(2011-340) (2011-340)

中国现代中药

OACSTPCD

1673-4890

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