中国现代中药2016，Vol.18Issue(3)：357-361,5.DOI:10.13313/j.issn.1673-4890.2016.3.023
六神曲炮制过程中微生物的分离与鉴定
Identification and Isolation of Microorganisms from Medicated Leaven during Its Production
摘要
Abstract
Objective:In order to elucidate the beneficial microorganisms in Medicated Leaven made by traditional fermented methods.Methods:Using classical microbial isolation and purification methods,the strains in Medicated Leaven samples were isolated and purified.The preliminary taxonomy of the strains isolated was carried out by investigating the morphological properties and analyzing and comparing their 16SrRNA or 18SrRNA gene sequence.Results:Eight species of bacteria,three species of yeasts and four species of filamentous fungi were isolated and identified in Medicated Leaven samples.The bacteria were Pantoea agglomerans, Staphylococcus haemolyticus, Enterobacter asburiae, Escherichia coli, Bacillus subtilis,Bacillus cereu, Cronobacter sakazakii,Pediococcus pentosaceus;the yeasts were Hyphopichia burtonii, Debaryomyces hansenii,Pichia kudriavzevii;the filamentous fungi were Mucor circinelloides,Mucor racemosus,Penicillium camemberti,Aspergillus candidus.Conclusion:Many species of microorganisms exist in Medicated Leaven samples,which help to reconstruct modern fermentation technology of Medicated Leaven.关键词
六神曲/发酵/微生物/分离/鉴定Key words
Medicated Leaven/fermentation/microorganism/isolation/identification引用本文复制引用
贾丹丹,黄春敏,刘英,胡海峰..六神曲炮制过程中微生物的分离与鉴定[J].中国现代中药,2016,18(3):357-361,5.基金项目
国家中医药管理局中医药行业专项(201507004) （201507004）
