中医药学报2016，Vol.44Issue(1)：52-54,3.
丹皮酚抑制 SiHa细胞增殖及 COX－2转录的实验研究
Inhibitory Effect of Paeonol on Proliferation and COX-2 Transcription in SiHa Cells
摘要
Abstract
Objective:To investigate the effect of Paeonol on the proliferation and COX -2 transcription of SiHa cells . Methods:Human cervical squamous cell carcinoma SiHa cell line was used as a model in vitro .The proliferation of SiHa cells treated by Paeonol was detected by MTT assays .Plasmids pCOX2-Luc and pAP1-Luc were transfected into SiHa cells and the dual luciferase reporter gene assay system was used to detect the transcription level of COX -2 and the ac-tivity of AP-1 .Results:The results of MTT assays showed that Paeonol significantly inhibited the proliferation of SiHa cells and the effect was associated positively with the dose and treatment time of paeonol .Luciferase assay results showed that Paeonol apparently reduced the transcription level of SiHa COX -2 cells ( P<0 .01 ) , and obviously inhibited the activity of AP-1(P<0.01).Conclusion:Paeonol may inhibit COX-2 transcription by depressing the activity of tran-scription factor AP-1,thereby suppress the proliferation of SiHa cells .关键词
丹皮酚/SiHa细胞/COX-2/AP-1Key words
Paeonol/SiHa cell/COX-2/AP-1分类
医药卫生引用本文复制引用
赵俊云,杨晓敏,郭健..丹皮酚抑制 SiHa细胞增殖及 COX－2转录的实验研究[J].中医药学报,2016,44(1):52-54,3.基金项目
北京市青年英才计划 （）
