中医药信息2016，Vol.33Issue(2)：43-46,4.
癃畅颗粒对大鼠前列腺增生组织 Survivin基因表达的影响
Effect of Longchang Granules on the Expression of Survivin Gene in Prostate Tissue of Benign Prostate Hyperplasia Rats
摘要
Abstract
Objective:To investigate the mechanism of Longchang granules in the treatment of benign prostatic hyperplasia.Methods:Divide 120 Wistar rats into 6 groups:normal group,model group,Longbishu group,low dose Longchang granule group , medium dose Longchang granule group and high dose Longchang granule group.Except normal group ,all of the rest rats were made into models of prostate hyperplasia by testosterone propionate injection after they were first castrated .All the model rats were killed after treated respectively with Longchang granules ,Longbishu and normal saline by gastrogavage once a day for 30 days.The apoptosis of cells was identified by TUNEL assay .IHC method was applied to detect the expression of Survivin gene in prostate tissue .Results:Compared with model group , the prostate apoptosis indexes of all treatment groups were all increased to different levels ,while the expression of Survivin protein reduced to deferent levels ( P﹤0.01 ) .No statistical significance could be found between high dose Longchang granule goup and Longbishu group(P﹥0.05).While a statistical significance could be found between medium ,low dose Longchang granule groups and Longbishu group ( P﹤0 .01 ) .There was a statistical significance among all groups of Longchang granules ( P﹤0 .01 ) .Conclusion:Longchang granules can downregulate the expression of Survivin gene in prostate tissue of benign prostate hyperplasia rats and accelerate cell poptosis .This is the possible mechanism of Longchang granules in the treatment of benign prostatic hyperplasia .关键词
癃畅颗粒/前列腺增生/存活素/细胞凋亡Key words
Longchang granules/Benign prostatic hyperplasia/Survivin/Cell apoptosis分类
医药卫生引用本文复制引用
朱帅,安立文,贾晓聪,杨珊珊,苑海刚,高山,马明,王斌,张景欣..癃畅颗粒对大鼠前列腺增生组织 Survivin基因表达的影响[J].中医药信息,2016,33(2):43-46,4.基金项目
黑龙江省教育厅科学技术研究面上项目(No.12531611) （No.12531611）
