安徽工程大学学报2016，Vol.31Issue(2)：21-25,39,6.
毕赤酵母产β－葡萄糖苷酶发酵条件优化
Optimization ofβGglucosidase fermentation conditions of Pichia pastoris
华骏 1杨帆 1李碧玲 1赵世光1
作者信息
- 1. 安徽工程大学 生物与化学工程学院，安徽 芜湖 241000
- 折叠
摘要
Abstract
Statistical method was used to optimize the fermentation ofβ-glucosidase produced by Pichia pastoris.Glycerol methanol and initial pH were confirmed as the critical factors impacting the enzyme activity according to the results of single factor experiment.Subsequently,by using response surface methodology,the optimum condition forβ-glucosidase yielding was obtained as following:1 9 .4 mL/L of glycerol,15 g/L of ammonium sulfate,2% of methanol with initial pH value of 6.12 and 20 mL of liquid volume.Theβ-glucosidase activity was up to 31.87 U/mL,which increased by 162.74% than that of the original.关键词
毕赤酵母/β-葡萄糖苷酶/发酵条件/响应面法Key words
Pichia pastoris/β-glucosidase/fermention condition/response surface methodology分类
化学化工引用本文复制引用
华骏,杨帆,李碧玲,赵世光..毕赤酵母产β－葡萄糖苷酶发酵条件优化[J].安徽工程大学学报,2016,31(2):21-25,39,6.
