安徽工程大学学报2016，Vol.31Issue(2)：31-35,5.
交联柚苷酶聚集体水解柚皮苷制备柚皮素
Hydrolysis of naringin to naringeninby crossGlinked naringinase aggregates
摘要
Abstract
In order to improve the naringenin yield,simplify the process of separation and purification and obtain the high purity production,the conversion of naringin to naringenin by cross-linked naringin aseaggregate was in-vestigated.The effects of substrate concentration,temperature,pH,enzyme amount and the reaction time on the preparation of naringenin were optimized,and the naringenin concentration and yield were used as the optimiza-tion indexes.Using the orthogonal design to optimize the hydrolysis process,the results showed that the feasible hydrolysis conditions were the substrate concentration 4.0 g/L,hydrolysis temperature at 55 ℃,pH 3.0,the a-mount of enzyme 18.0 mg/mL,the reaction time for 24 h,under which the production of naringenin's concentra-tion was 1.833 g/L and the yield was 97.72%.It provided a new method for preparing the naringenin.关键词
交联柚苷酶聚集体/柚皮素/水解反应/正交试验Key words
cross-linked naringinase aggregate/naringenin/hydrolysis reaction/orthogonal design分类
化学化工引用本文复制引用
朱必玉,钱伟,王先锋,杜平,高乾坤,魏胜华..交联柚苷酶聚集体水解柚皮苷制备柚皮素[J].安徽工程大学学报,2016,31(2):31-35,5.基金项目
国家级大学生创新创业训练计划基金资助项目(201310363075) （201310363075）
