北京大学学报（医学版）2016，Vol.48Issue(1)：1-4,4.DOI:10.3969/j.issn.1671-167X.2016.01.001
基于人胚胎干细胞健康安全评价体系的构建
Development of human embryonic stem cell platforms for human health-safety evaluation
摘要
Abstract
SUMMARY Thehumanembryonicstemcells(hESCs)serveasaself-renewable,genetically-healthy, pluripotent and single source of all body cells,tissues and organs.Therefore,it is considered as the good standard for all human stem cells by US,Europe and international authorities.In this study,the standard and healthy human mesenchymal progenitors,ligament tissues,cardiomyocytes,keratinocytes,primary neurons,fibroblasts,and salivary serous cells were differentiated from hESCs.The human cellular health-safety of NaF,retinoic acid,5-fluorouracil,dexamethasone,penicillin G,adriamycin,lead ace-tate PbAc,bisphenol A-biglycidyl methacrylate (Bis-GMA)were evaluated selectively on the standar-dized platforms of hESCs,hESCs-derived cardiomyocytes,keratinocytes,primary neurons,and fibro-blasts.The evaluations were compared with those on the currently most adopted cellular platforms.Parti-cularly,the sensitivity difference of PM2.5 toxicity on standardized and healthy hESCs derived fibroblasts, currently adopted immortalized human bronchial epithelial cells Beas-2B and human umbilical vein endo-thelial cells (HUVECs)were evaluated.The results showed that the standardized hESCs cellular plat-forms provided more sensitivity and accuracy for human cellular health-safety evaluation.关键词
胚胎干细胞/毒性试验/细胞分化/体外研究/人类Key words
Embryonic stem cells/Toxicity tests/Cell differentiation/In vitro/Humans分类
医药卫生引用本文复制引用
俞光岩,肖苒,欧阳宏伟,彭晖,陈晓,赵增明,王晓颖,方海琴,路璐,任玉兰,徐明明,曹彤,邹晓晖,张学慧,傅歆,彭双清,邓旭亮,李盛林,刘鹤..基于人胚胎干细胞健康安全评价体系的构建[J].北京大学学报（医学版）,2016,48(1):1-4,4.基金项目
科技部国际科技合作项目（2011 DFA32190）资助 Supported by the International Scientific and Technical Cooperation Pragram of China （）
