北京大学学报（医学版）2016，Vol.48Issue(1)：63-70,8.DOI:10.3969/j.issn.1671-167X.2016.01.011
不同纤维直径聚吡咯通电后对细胞和细菌黏附增殖的影响
Effects of different fibre diameter of polypyrrole after direct current stimulation on adhesion and proliferation of cells and bacteria
摘要
Abstract
Objective:To study the effects of cells ’ and bacteria ’s adhesion and proliferation on different fiber diameters of polypyrrole coating with electricity.Methods:Titanium coated with polypyr-role was divided into no electrical stimulation and stimulation groups,each group had 30-60 nm,70-1 00 nm,1 30-1 70 nm diameters of the fiber.MC3 T3 cells and Staphylococcus aureus (S.aureus)were inoculated on different fiber diameters of polypyrrole coating with and without electric stimulation .We gave the electrical stimulation group 1 00 mV for 1 h and every 24 hours gave it 1 h stimulation,and no e-lectrical stimulation group was not managed.We used scanning electron microscope (SEM)to observe the cells’and bacteria’s morphology.The cells were given 20 mL CCK-8 solutions after 1 ,3,7 days’ cultivation,then incubated for 2 h,the solution was transferred to 96-well plate,we measured the cells’ CCK-8 of the 30-60 nm,70-1 00 nm,and 1 30-1 70 nm groups by Elisa.The cells on different fiber diameters were also stained by live-dead cell staining kit,TritonX-1 00 and DAPI.We used PBS to wash and glycerin to seal them.The live-dead situation and morphology were tested by co focal microscope. The bacterial were stained by Live/dead baclight bacterial viability kits,we detect the suspension’s D of the 30-60 nm,70-1 00 nm,and 1 30-1 70 nm groups,and also observed the bacteria’s survival situa-tion by co focal microscope.Results:The CCK-8 of the cells with direct current stimulation was higher than that of the unpowered group (F=1 2.248,P=0.006).The smaller the fiber diameter,the better was the cell’s adhesion and proliferation (F=9.261 ,P=0.005).The bacterial suspension’s D of the electric group was lower than that of the unpowered group,and the fiber diameter had no significant effect on the bacteria’s growth(F=9.641 ,P=0.036).Conclusion:Polypyrrole coating with electricity can promote the cell’s proliferation and inhibit the bacteria’s proliferation,and the cell growth on small fiber diameter coating is better.There is no difference in the bacterial growth of different fiber diameter coatings.关键词
聚吡咯/直流电刺激/细胞增殖/细菌黏附Key words
Polypyrrole/Direct current stimulation/Cells proliferation/Bacteria adhesion分类
医药卫生引用本文复制引用
杨颖婷,卫彦,夏斌,王珍高..不同纤维直径聚吡咯通电后对细胞和细菌黏附增殖的影响[J].北京大学学报（医学版）,2016,48(1):63-70,8.基金项目
国家重点基础研究发展计划（973计划，2012CB93390）、北京市自然科学基金（7144256）和北京市科学技术委员会首都特色应用研究项目（Z141107002514058）资助Supported by the National Basic Research Program of China （973 Program，2012CB93390），Beijing Natural Science Foundation （7144256）and the Capital Foundation for Clinical Characteristics and Application Research （）
