动物医学进展2016，Vol.37Issue(4)：18-23,6.
大鼠成熟的肺表面活性蛋白 B 的原核表达及纯化
Prokaryotic Expression and Purification of Mature Pulmonary Surfactant Protein B of Rats
摘要
Abstract
In order to express and purify surfactant protein B(SP-B),and the gene of SP-B from rat was cloned,constructed into the prokaryotic vector pGEX4T-1.Firstly,rare codons of SP-B gene were opti-mized,and cloned into linear pGEX4T-1 after PCR.Secondly,the recombinant plasmid was transformed in-to E.coli BL21(DE3),and the expression of SP-B was detected by SDS-PAGE and Western blot.Then,the recombinant protein was purified by GSTPrep FF 16/10.The results showed that the SP-B gene was 250 bp and the result of sequencing pGEX4T-1/SP-B was consistent with the SP-B cDNA sequence.At the same time,the E.coli BL21(DE3)was induced by IPTG,and one new band about 34 ku appeared.Those results indicated that the pGEX4T-1/SP-B recombinant plasmid was constructed successfully,and the ex-pression and purification of SP-B could be the foundation of pulmonary surfactant alternative drugs.关键词
肺表面活性蛋白 B/原核表达/免疫印迹/蛋白纯化/大鼠Key words
surfactant protein B/prokaryotic expression/Western blot/protein purification/rat分类
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张潇骏,刘大维,王万能,周继红,刘钦,顾仕苓,周代君,邱俊,代维,袁丹凤..大鼠成熟的肺表面活性蛋白 B 的原核表达及纯化[J].动物医学进展,2016,37(4):18-23,6.基金项目
国家自然科学基金项目 （）
