南方医科大学学报2016，Vol.36Issue(4)：572-576,581,6.
雷帕霉素对大鼠主动脉瓣膜间质细胞增殖的影响
Effect of rapamycin on proliferation of rat heart valve interstitial cells in vitro
摘要
Abstract
Objective To investigate the effect of rapamycin on the proliferation of rat valvular interstitial cells in primary culture. Methods The interstitial cells isolated from rat aortic valves were cultured and treated with rapamycin, and the cell growth and cell cycle changes were analyzed using MTT assay and flow cytometry, respectively. RT-PCR was used to detect mRNA expression levels of S6 and P70S6K in cells, and the protein expressions level of S6, P70S6K, P-S6, and P-P70S6K were detected using Western blotting. Results Rat aortic valvular interstitial cells was isolated successfully. The rapamycin-treated cells showed a suppressed proliferative activity (P<0.05), but the cell cycle distribution remained unaffected. Rapamycin treatment resulted in significantly decreased S6 and P70S6K protein phosphorylation level in the cells (P<0.05). Conclusion The mechanism by which rapamycin inhibits the proliferation of valvular interstitial cells probably involves suppression of mTOR to lower S6 and P70S6K phosphorylation level but not direct regulation of the cell cycle.关键词
mTOR/雷帕霉素/瓣膜间质细胞/细胞增殖Key words
mammalian target of RAPA/rapamycin/aortic valvular interstitial cells/cell proliferation引用本文复制引用
谭焱,王继业,易仁亮,邱健..雷帕霉素对大鼠主动脉瓣膜间质细胞增殖的影响[J].南方医科大学学报,2016,36(4):572-576,581,6.基金项目
广东省自然科学基金（2014A030313598）；广州市科技计划 （）
