森林与环境学报2016，Vol.36Issue(2)：155-159,5.DOI:10.13324/j.cnki.jfcf.2016.02.005
液体培养过程中金花茶体细胞胚增殖优化
Optimization of propagation of somatic embryos from Camellia nitidissima in liquid medium
摘要
Abstract
For obtaining of a large number of consistent somatic embryos in Camellia nitidissima, single factor experiment design and complete randomized experiment design were carried out to observe the effects of carbon source combination, proline content and medium volume together with initial inoculum on the propagation of them in liquid medium one after another. Results showed as follows. The type of carbon source combination had no significant effect on the proliferation of somatic embryos but aberration rate of somatic embryos was much lower in the medium supplented with 40 g·L-1 saccharose together with 20 g·L-1 sorbitol compared with the other two treatments. The proline content had an extremely significant effect on the proliferation of somatic embryos and its optimal content was 2 g·L-1 . Medium volume, initial inoculum and interaction between them all affected the proliferation of somatic embryos extremely significantly and among them fluid volume was the most significant factor. The optimal medium volume was 45 mL. The optimal initial inoculum was 0. 5 or 1. 0 g. The multiplication rates of somatic embryos cultured under best treatment combination was as much as 5.65 and their consistency was good.关键词
金花茶/体细胞胚/液体培养/碳源/脯氨酸/培养基体积/接种量Key words
Camellia nitidissima Chi/somatic embryos/liquid culture/carbon source/proline/medium volume/initial inoculum分类
农业科技引用本文复制引用
钟春水,赖瑞联,刘生财,赖钟雄..液体培养过程中金花茶体细胞胚增殖优化[J].森林与环境学报,2016,36(2):155-159,5.基金项目
福建省科技厅农业科技平台建设项目(2008N2001)。 （2008N2001）
