湖南中医药大学学报2016，Vol.36Issue(4)：1-5,5.DOI:10.3969/j.issn.1674-070X.2016.04.001
丹龙醒脑方对脑缺血再灌注大鼠半暗带区神经细胞凋亡与即早基因表达的影响
Effects of Danlong Xingnao Prescription on Neural Cell Apoptosis and Expression of Immediate Early Gene in Schemic Penumbra of Rats with Cerebral Ischemia-Reperfusion Injury
摘要
Abstract
〔Abstract〕 Objective To investigate the relationship of Danlong Xingnao Prescription (DLXNP) on neural cell apoptosis and expression of c-fos, c-jun and c-myc in ischemic penumbra of rats with cerebral ischemia-reperfusion injury (MCAO/R). Methods 48 male SD rats were randomly divided into the sham-operation group, cerebral ischemi a-r eperfusion injury (model) group, nimodipine group, small, medium, high dose of DLXNP groups. The middle cerebral artery occlusion/ reperfusion models were prepared by suture method. The neural function defect scale was evaluated after 24 hours of reperfusion, researchers carried out, and the ischemic side of brain cortex was taken out. The nerve cells of apoptosis were detected by using terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) method, and the expression of c-fos, c-jun and c-myc was determined by immunohistochemistry. Results Compared with the operation group, the neural function defect scale, apoptotic index (AI) and integrated optical density (IOD) of c-fos, c-jun, c-myc were increased, the differences were statistically significant (P<0.05, P<0.01). Compared with model group, the neural function defect scale, AI and IOD of c-fos, c-jun, c-myc in nimodipine group and DLXNP groups were reduced (P<0.05, P<0.01); The defferences between nimodipine groupand different doses of DLXNP groups were not statistically significant in all indicators (P>0.05). Conclusion Danlong Xingnao pecipe can lower the neural function defect and reduced neural cell apoptosis, which may relate to the down-regulated expression of c-fos, c-jun and c-myc.关键词
丹龙醒脑方/脑缺血再灌注/神经细胞凋亡/即早基因Key words
Danlong Xingnao prescription/cerebral ischemia reperfusion/neural cell apoptosis/immediate early genes分类
医药卫生引用本文复制引用
曹泽标,陈昱文,刘旺华,周小青,陈娉婷,李花..丹龙醒脑方对脑缺血再灌注大鼠半暗带区神经细胞凋亡与即早基因表达的影响[J].湖南中医药大学学报,2016,36(4):1-5,5.基金项目
国家自然科学基金项目（81373702，81473567，81202632）；教育部博士点基金项目（20124323120003）；湖南省自然科学基金（13JJ3097）；湖南省教育厅科研项目（14B134，15K092）；湖南中医药大学“一方”研究生创新项目（2015YF02）。 （）
