湖南中医药大学学报2016，Vol.36Issue(4)：6-10,5.DOI:10.3969/j.issn.1674-070X.2016.04.002
中药复方提取物HNA-1对SIV慢性感染恒河猴胸腺输出及胸腺细胞发育的影响
Effects of HNA-1 Extracted from a Chinese Medicine Compound on the Thymic Output and Thymocyte Development in Rhesus Macaques with SIV Chronic Infection
摘要
Abstract
〔Abstract〕 Objective To investigate the effect of Chinese herbal extract HNA-1 on HIV-1 infection and AIDS. Methods Eight Chinese rhesus macaques had been infected by SIVmac239 for 16 to 21 months were enrolled into this study, the treatment group received HNA-1, control group received normal saline for consecutively 2 months, respectively. In predesigned time-points, peripheral blood were drawn for DNA extraction, and thereby thymic output was evaluated by T-cell receptor excision circle (TRECs) quantification. The thymocyte cells were seperated by enzyme digestion, the proportions of CD34 + cells and thymocytes in various development stages were analyzed by flow cytometry. Results Compared with control group, the thymic output function obviously was elevated in HNA-1 group (P<0.05). And the proportion of CD34+Lin-cells was significantly increased (P <0.05). More interestingly, the thymocytes in double positive (DP) stageⅡdecreased dramatically, while thymocytes in stage DP Ⅲ and stage DP Ⅳ increased. Conclusion Enhanced thymic output function may contribute to the effect of HNA-1 on AIDS treatment with underlying mechanisms involving increased thymic progenitor cells and promoted development of thymocytes from stage DPⅡ to stage DP Ⅲ.关键词
湘A-号方/CD4+T 细胞/胸腺输出/胸腺细胞发育Key words
HNA-1/CD4+T cells/thymic output/thymocyte development分类
医药卫生引用本文复制引用
王小妹,陈颂,陈滢宇,胡燕,张晓宇,宁兴旺,朱惠斌..中药复方提取物HNA-1对SIV慢性感染恒河猴胸腺输出及胸腺细胞发育的影响[J].湖南中医药大学学报,2016,36(4):6-10,5.基金项目
国家自然科学基金资助项目(81274169)；湖南省高等学校科学研究重点项目(12A102)；湖南省教育厅项目（13C688）；湖南省高校科技创新团队“感染性疾病中医药防治研究”资助项目(NO:15)。 （81274169）
