军事医学2016，Vol.40Issue(4)：294-298,5.DOI:10.7644/j.issn.1674-9960.2016.04.007
利用骨髓间充质干细胞膜片原位构建“三明治”样组织工程牙周膜的实验研究
Periodontal regeneration on surface of tooth root using BMSCs sheet combined with a sandwich structure:a canine model in situ
摘要
Abstract
Objective To probe the periodontal ligament regeneration following the implantation of bone marrow mesenchymal cells ( BMSCs ) sheet-collagen membrane-BMSCs sheet sandwich complex.Methods BMSCs cell sheet-collagen membrane-BMSCs sheet complexes were compounded on the root surface of teeth of Beagle dogs.All the dogs were killed on 4 and 12 weeks after implantation.Periodontal ligament regeneration was observed by radiological means, HE staining and Sirus-red staining.Results Compared with collagen membrane group and blank control group, there was a clearly periodontal ligament like tissue and Sharpey′s like fibres formation in test group only.Conclustion Cell sheet-collagen membrane-cell sheet sandwich complex can effectively improve the periodontal ligament regeneration.关键词
骨髓间充质干细胞/细胞膜片/牙周膜再生/细胞外基质/组织工程Key words
bone marrow mesenchymal stem cell/cell sheet/periodontal ligament regeneration/extracellular matrix/tissue engineering分类
医药卫生引用本文复制引用
刘翠,朱彪,张欣然,刘东华,赵刚,郭希民,郭红延..利用骨髓间充质干细胞膜片原位构建“三明治”样组织工程牙周膜的实验研究[J].军事医学,2016,40(4):294-298,5.基金项目
国家自然科学基金面上资助项目 （）
