泸州医学院学报2016，Vol.39Issue(2)：114-117,4.DOI:10.3969/j.issn.1000-2669.2016.02.002
β-葡聚糖对巨噬细胞摄取氧化低密度脂蛋白的影响
Effects of β-glucan on the uptake of oxLDL in macrophages
摘要
Abstract
Objective: To explore the effects and the mechanisms of β-glucan on the formation of macrop-hage-derived foam cells. Methods: The effects of β-glucan on cell viability was examined by MTT. Oil-red O staining was used to observe the effects ofβ-glucan on the uptake of oxLDL,and the effects ofβ-glucan on SR-A、CD36、c-fos、c-Jun protein expression in macrophages were investigated using Western blot. Data were analyzed using SPSS 10.0 statistical software and two-tailed t test was used for statistical significance assessment. Results: β-glucan ranged from 2.5 ~ 10 μg /mL did not affect cell viability, and at 5 μg/mL inhibited the formation of macrophage-derived foam cells via attenuating oxLDL uptake by macrophages. Compared with control group, β-glucan (2.5 ~ 10 μg /mL) treatment dose-dependently decreased the expression of SR-A and nuclear c-Jun without affecting that of CD36 and nuclear c-fos in macrophages. Compared with β-glucan at 5 μg/mL group, SP600125(c-Jun inhibitor) significantly augmented the inhibitory effects ofβ-glucan on SR-A expression(P< 0.05). Conclusion: β-glucan could inhibit the formation of macrophage-derived foam cells, which are possibly related to the inactivation of c-Jun/SR-A pathway.关键词
动脉粥样硬化/细胞模型/β-葡聚糖/巨噬细胞/泡沫细胞Key words
Athrosclerosis/Cell model/β-glucan/Macrophage/Foam cells分类
医药卫生引用本文复制引用
李秀英,叶云..β-葡聚糖对巨噬细胞摄取氧化低密度脂蛋白的影响[J].泸州医学院学报,2016,39(2):114-117,4.基金项目
国家青年自然科学基金 （）
