泸州医学院学报2016，Vol.39Issue(2)：118-123,6.DOI:10.3969/j.issn.1000-2669.2016.02.003
红景天苷对人乳腺癌MDA-MB-435细胞功能的作用
Effect of Salidroside on the function of human breast cancer cell line MDA-MB-435
摘要
Abstract
Objective: To explore the effects and mechanisms of Salidroside on human breast cancer. Human breast cancer cell line MDA-MB-435 was treated with Salidroside (Sal), and cell proliferation, apoptosis, cell migration and invasion were evaluated. Methods: The MDA-MB-435 cells were cultured in vitro. The half maximal inhibitory concentration (IC50) of Salidroside on MDA-MB-435 cells was determined by CCK-8 assay. MDA-MB-435 cells treated with IC50 of Sal were considered as the Sal group, and cells without Sal treatment were as the control group. Cell morphology was observed using light microscope, cell proliferation was detected by CCK-8 assay, apoptosis and cell cycle were evaluated by flow cytometry, and cell migration and invasion were studied using Transwell assay. Results:The IC50 of Sal on human breast cancer MDA-MB-435 cells was 4 mg/mL. The cells treated with Sal showed the typical morphology of necrosis and apoptosis under light microscropy. Using CCK-8 assay, cells proliferation was significantly inhibited at the IC50 of Sal at 24 h, 48 h and 72 h, relative to the control group (P < 0.05), and the inhibition showed a time-dependent manner. Flow cytometry using AnnexinⅤ-FITC/PI apoptosis assay kit demonstrated that the percentage of early apoptotic cells was significantly increased(3.31±0.21)%in the Sal group, compared to(1.26± 0.13)% in the control group(P< 0.05), and the late apoptosis and necrosis was also significantly induced (28.80 ± 2.15)% in the Sal group, compared to (5.56±0.78)%in the control group (P<0.01). The results of FITC demonstrated that the ratio of G0/G1 was increased, while the cells at S phase and the ratio of G2/M were decreased compared to those of the control group. The cell migration and invasion assay revealed that the average number of invaded and migrated cells in the Sal group in migration assay (39.60 ± 3.37), and in invasive assay (4.20 ± 1.55) were significantly decreased compared to the control group in migration assay (142.20 ± 12.99) (P< 0.01) and in invasive assay(13.00 ± 3.09) (P < 0.05), respectively. Conclusion: Salidroside treatment significantly inhibited cell proliferation, and promoted apoptosis, arrested cell cycle and repressed the motility and invasion of MDA-MB-435 cells.关键词
红景天苷/乳腺癌/增殖/凋亡/侵袭Key words
Salidroside/Breast cancer/Proliferation/Apoptosis/Invasion分类
医药卫生引用本文复制引用
龚舒,段承刚,陶忠桦,刘晓燕,傅俊江,甘淋..红景天苷对人乳腺癌MDA-MB-435细胞功能的作用[J].泸州医学院学报,2016,39(2):118-123,6.基金项目
四川省科技厅应用基础研究计划项目 （）
