泸州医学院学报2016，Vol.39Issue(2)：124-127,4.DOI:10.3969/j.issn.1000-2669.2016.02.004
氧化应激对静脉性溃疡SDF-1α/CXCR4时相性表达的影响
Effect of oxidative stress on the expression of SDF-1α/CXCR4 in venous ulcer
钟武 1杨帆1
作者信息
- 1. 西南医科大学附属医院急诊医学部，四川泸州 646000
- 折叠
摘要
Abstract
Objective: To investigate the effect of oxidative stress on the expression of SDF-1α/CXCR4 in venous ulcer. Methods: Ulcer samples were obtained from 56 patients including 40 males and 16 females with lower extremity venous ulcer treated. The ulcer samples were divided into 2, 3, 4, and 5 weeks groups based on the duration of the disease, and normal skin samples from 56 patients were used as controls. The oxidative stress products, MDA and GSH were analyzed. The expression of SDF-1α and CXCR4 was analyzed by western blot followed by densitometry analysis, and the colocalization of SDF -1α and CD31 was evaluated by immunofluorescence double staining. Results: After 3 weeks the concentration of MDA increased, whereas that of GSH decreased significantly (P< 0.05) respe-ctively compared to controls. The expression of both SDF-1α and CXCR4 was gradually decreased, which became significant (P < 0.05) after 3 weeks compared with that in the control groups. Immunofluorescence double staining revealed that the level of SDF-1α and CD31 was also markedly decreased in the venous ulcer group after 3 weeks compared with those in the control group, SDF-1αand CD31 colocalization was observed. Conclusion The decreased expression of SDF-1α/CXCR4 induced by oxidative stress is possibly associated with the pathogenesis of venous ulcer.关键词
基质细胞衍生因子-1α/趋化因子受体4/静脉性溃疡/氧化应激Key words
Stromal cell derived factor-1α/Chemokine recceptor 4/Venous ulcer/Oxidative stress分类
医药卫生引用本文复制引用
钟武,杨帆..氧化应激对静脉性溃疡SDF-1α/CXCR4时相性表达的影响[J].泸州医学院学报,2016,39(2):124-127,4.
