摘要
Abstract
Objective: To evaluate the protective effect of Edaravone on brainstem after ischemia/reperfusion injury and to provide experimental evidence for clinic, rats were pretreated with Edaravone and its effect on MDA and SOD were studied. Methods: 25 SD rats used in experimental animals were divided into 3 groups at random:sham operation group,brainstem ischemia reperfusion group and Edaravone pretreatment group(n = 10). Accord-ing to different ischemia time period, rats in ischemia/reperfusion group and Edaravone pretreatment group were further divided evenly into ischemia 1 h/reperfusion 7 h and ischemia 3 h/reperfusion 5 h subgroups, respectively. The 1st and the 2nd basilar artery without branch areas were exposed, and animals were observed without any treatment for 8 h in normal group, the arteries were clamped with Micro-bulldog clamp to create ischemia for 1 h/reperfusion 7 h (5 animals) and ischemia for and 3 h/reperfusion 7 h (5 animals) respectively in ischemia reperfusion group, and animals in Edaravone pretreatment group were given Edaravone (6 mg/kg) by tail vein injection 10 min before ischemia/reperfusion as described in ischemia/reperfusion group. The histology of brainstems after HE staining were compared, the brainstem tissues at various stages was observed by light micro-scope at 200 magnification. The activities of SOD and the contents of MDA at various subgroups were measured with spectrophotometry. Results: When the time on ischemia 1 h, compared with the normal group and pretreat-ment group, the contents of MDA were significantly increased and the contents of SOD were obviously decreased in ischemia reperfusion group, the differences all had statistical significance (P< 0.05). When the time on is-chemia 3 h, compared with the normal group , the contents of MDA were significantly increased and the contents of SOD were obviously decreased in pretreatment group and ischemia reperfusion group, the differences all had statistical significance(P<0.05);Compared with the pretreatment group, the contents of MDA were significantly increased and the contents of SOD were obviously decreased in ischemia reperfusion group, the differences all had statistical significance (P < 0.05). Conclusion: Edaravone played a tremendous and definite role in ischemia reperfusion injury of brainstem in rats. Its mechanism has to do with the reduced levels of MDA, the increased activity of SOD, the removal of oxyradicals, and the inhibited overoxidation of lipid.关键词
依达拉奉/预处理/脑干缺血再灌注损伤/MDA/SODKey words
Edaravone/Pretreatment/Brainstem ischemical reperfusion/MDA/SOD分类
医药卫生
