山东医药2016，Vol.56Issue(13)：10-12,3.DOI:10.3969/j.issn.1002-266X.2016.13.004
黄芩素对人结肠癌细胞增殖、凋亡、侵袭的影响及机制
Effects of baicalein on proliferation, apoptosis and invasion of human colorectal cancer cells
摘要
Abstract
Objective To investigate the effects of baicalein on proliferation, apoptosis and invasion of human color-ectal cancer cells and to explore the mechanism.Methods Human colorectal cancer cells SW480 in logarithmic phase were randomly divided into the control group, 25, 50 and 100 μmol/L baicalein treatment groups which were respectively treated with normal saline, 25, 50 and 100μmol/L baicalein.The effects of baicalein on proliferation, apoptosis and inva-sion of SW480 cells were detected by EdU, MTT, flow cytometry and Transwell test.The protein expression of Bcl-2, Bax and vascular endothelial growth factor ( VEGF) in SW480 cells after the treatment of baicalein was detected by Western blotting.Results In the 25, 50 and 100μmol/L baicalein treatment groups, the proliferation of SW480 cells was inhibited with a dose and time-dependent manner, the apoptosis rates of SW480 cells were gradually increased, and the invasion a-bilities of SW480 cells were gradually decreased.After the treatment of baicalein, the expression of Bcl-2 and VEGF in SW480 cells was down-regulated, and the expression of Bax was up-regulated (P<0.05).Conclusion Baicalein inhibits the proliferation and invasion of SW480 cells and promots the apoptosis, which might be related with the down-regulation of Bcl-2 and VEGF expression and the up-regulation of Bax expression.关键词
结肠肿瘤/黄芩素/细胞增殖/细胞凋亡/血管内皮生长因子Key words
colorectal neoplasms/baicalein/cell proliferation/apoptosis/vascular endothelial growth factor分类
医药卫生引用本文复制引用
王宁,程琦,张延新..黄芩素对人结肠癌细胞增殖、凋亡、侵袭的影响及机制[J].山东医药,2016,56(13):10-12,3.基金项目
河南省教育厅青年教师资助项目（2012GGJS-269）；漯河医学高等专科学校科学研究项目（2010S06）。 （）
