山东医药2016，Vol.56Issue(13)：13-16,4.DOI:10.3969/j.issn.1002-266X.2016.13.005
四种重楼皂苷对结肠癌细胞的体外毒性作用比较
In vitro toxicity of four kinds of polyphyllins on colon cancer cells
庞晓辉 1王朝杰 1史祖宣 1崔瑶 1崔永霞 1高天慧1
作者信息
摘要
Abstract
Objective To investigate the in vitro toxicity of polyphyllins I, V, VI and H on colon cancer cells and to provide guidance for seeking effective chemotherapy drugs of colon cancer.Methods The resurgent SW620, SW480, Co-lo320, Lovo colon adenocarcinoma cell lines were co-cultured with polyphyllin I, polyphyllin VI, polyphyllin H, polyphyl-lin V and oxaliplatin for 48 h respectively, which were then added with CCK-8 for two-hour incubation.The optical density at 450 nm was detected by microplate reader.The cytotoxicity of polyphyllin on colon cancer cells was analyzed by SPSS 23.0.Annexin V/PI staining and flow cytometry was used to detect the apoptotic cells.Western blotting was applied to de-tect Bax and Caspase-3 protein expression.Results The polyphyllin I, polyphyllin VI, polyphyllin H and polyphyllin V showed remarkable antineoplastic activity on SW620, SW480, Colo320 and Lovo cell lines with IC50 of 0.4 mg/L to 8 mg/L.The anticancer activity of polyphyllin I and polyphyllin V were stronger than that of polyphyllin VI and polyphyllin H ( P<0.05).The in vitro anti-tumor effect of polyphyllin I with IC50 of 0.4-0.6 mg/L was the same as oxaliplatin with IC50 of 0.1-2.1 mg/L.Flow cytometry demonstrated that the percentage of Annexin V/PI positive cells treated with polyphyllin I increased 52.3%.Western blotting showing that polyphyllin I mediated expression of Bax and Caspase-3 in colorectal cells was 3 and 1.13 times higher than that of the control group.Conclusion Polyphyllin I, polyphyllin VI, polyphyllin H and polyphyllin V show good anticancer activity against colon adenocarcinoma cell lines in vitro, especially polyphyllin I which can mediate the apoptosis of colon cancer cells.关键词
结肠癌/重楼皂苷/细胞凋亡Key words
colorectal carcinoma/polyphyllin/apoptosis分类
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庞晓辉,王朝杰,史祖宣,崔瑶,崔永霞,高天慧..四种重楼皂苷对结肠癌细胞的体外毒性作用比较[J].山东医药,2016,56(13):13-16,4.
