山东医药2016，Vol.56Issue(13)：17-19,3.DOI:10.3969/j.issn.1002-266X.2016.13.006
三七总皂苷对重症急性胰腺炎大鼠的治疗作用及机制
Effect of panax notoginseng saponins in treatment of rats with severe acute pancreatitis
摘要
Abstract
Objective To investigate the effect of panax notoginseng saponins in treatment of rats with severe acute pancreatitis ( SAP) , and to explore the mechanism.Methods Thirty SD rats were randomly divided into three groups:sham operation group ( SO group ) , severe acute pancreatitis group ( SAP group ) , PNS preconditioning group ( PNS group) , and there were 10 rats in each group.Rat SAP models were induced by retrograde injection of 5%taurocholic acid sodium to pancreatic duct.SO group was just treated with pancreas and duodenum turning after abdominal closure without injection of 5%taurocholic acid sodium.The rats in the PNS group were given intraperitoneal injection of 0.1 mL/100 g PNS 50 mg/mL (xuesetong injection 0.1 mL/100 g) after the models were established.And rats in the other two groups were given normal saline (0.1 mL/100 g) .The serum AMS were detected at 4 hours after modeling, we observed the path-ological changes of pancreatic tissues under the light microscopy.The expression of nuclear transcription factor-κB ( NF-κB) and tumor necrosis factor-α( TNF-α) was detected by immunohistochemical SP method.Results Compared with the group SO, serum AMS levels, pathology scores of pancreatic tissues and the expression of NF-κB and TNF-αwere signifi-cantly increased in the groups PNS and SAP ( all P<0.05) , and those in the SAP group rats were significantly higher than those in the PNS group (all P<0.05).The expression of NF-κB was positively correlated with TNF-αexpression in the pancreas acinar cells (r=0.958, 0.992 and 0.989, all P<0.05).Conclusions Panax notoginseng saponins may reduce the pancreatic injuries of SAP rats.Panax notoginseng saponins may have protective effect on SAP rats by inhibiting the ac-tivation of NF-κB and reducing the excessive release of inflammatory factors.关键词
重症急性胰腺炎/三七总皂苷/核转录因子κB/肿瘤坏死因子αKey words
severe acute pancreatitis/panax notoginseng saponins/nuclear transcription factor-κB/tumor necrosis factor-α分类
医药卫生引用本文复制引用
盛国涛,李双喜,莫小华..三七总皂苷对重症急性胰腺炎大鼠的治疗作用及机制[J].山东医药,2016,56(13):17-19,3.基金项目
昆明医科大学联合专项基金资助项目（2007C138M）；昆明医科大学研究生创新基金资助项目（2015S91）。 （）
