中国组织工程研究2016，Vol.20Issue(10)：1433-1438,6.DOI:10.3969/j.issn.2095-4344.2016.10.009
SD大鼠骨髓间充质干细胞在-80℃低温条件下的冻存
Cryopreservation of bone marrow mesenchymal stem cells from Sprague-Dawley rats at-80℃
陈凯 1李欣然 2宣言3
作者信息
- 1. 河北工程大学农学院，河北省邯郸市 056021
- 2. 驻马店市驿城区农业局，河南省驻马店市 463000
- 3. 邯郸市第一中学，河北省邯郸市 056001
- 折叠
摘要
Abstract
BACKGROUND:We attempt to explore a low-cost, simple and effective way to cryopreserve bone marrow mesenchymal stem cels at-80℃. <br> OBJECTIVE:To screen the optimal cryopreservation fluid for bone marrow mesenchymal stem cels and to verify the biological features of bone marrow mesenchymal stem cels after long-term cryopreservation. <br> METHODS: Bone marrow mesenchymal stem cels were cultured using adherent method and the biological features and purity of cels were detected using immunofluorescence method. Bone marrow mesenchymal stem cels were cryopreserved in the cryoprotectant medium containing low-sugar DMEM, fetal bovine serum and dimethyl sulfoxide at different proportions at-80℃ for a short term. Then, the optimal cryoprotectant was selected to storage the bone marrow mesenchymal stem cels. After 1, 3, 6 months of cryopreservation, the cels were resuscitated, cultured and passaged. Passage cels were identified immunofluorescence method to determine the biological features of bone marrow mesenchymal stem cels cryopreserved at-80℃. <br> RESULTS AND CONCLUSION:Cryoprotectant medium of 80% DMEM+10% fetal bovine serum+10% dimethyl sulfoxide was suitable for cryopreserving MSCs at -80℃, and resuscitated cels were able to proliferate in vitro, and passage normaly, indicating the cryopreserved bone marrow mesenchymal stem cels stil maintain the original biological activity.关键词
干细胞/骨髓干细胞/细胞培养/骨髓间充质干细胞/二甲基亚砜/冻存保护液Key words
Bone Marrow/Mesenchymal Stem Cels/Dimethyl Sulfoxide/Cryopreservation/Tissue Engineering分类
医药卫生引用本文复制引用
陈凯,李欣然,宣言..SD大鼠骨髓间充质干细胞在-80℃低温条件下的冻存[J].中国组织工程研究,2016,20(10):1433-1438,6.
