中国组织工程研究2016，Vol.20Issue(11)：1544-1550,7.DOI:10.3969/j.issn.2095-4344.2016.11.004
三维环境下软骨细胞诱导滑膜间充质干细胞向软骨样细胞的分化
Chondrocyte-like differentiation of synovial mesenchymal stem cells co-cultured with chondrocytes on a three-dimensional scaffold
摘要
Abstract
BACKGROUND:The co-culture of chondrocytes and synovial mesenchymal stem cels can induce the cartilage differentiation of synovial mesenchymal stem celsin vitro, but the cel differentiation induced by co-culturein vivo is rarely reported. <br> OBJECTIVE:To investigate the chondrogenic differentiation of synovial mesenchymal stem cels co-cultured with chondrocytes on the chitosan/type I colagen composite scaffolds after being transplanted into the subcutaneous layer of Sprague-Dawley rats. <br> METHODS: The synovial mesenchymal stem cels and chondrocytes harvested from the synovial membrane and articular cartilage of Sprague-Dawley rats were obtained by enzyme digestion method and cultured respectively. Passage 3 synovial mesenchymal stem cels and passage 2 chondrocytes, which were divided into four groups: group A (chondrocytes alone), group B (synovial mesenchymal stem cels alone), group C (ratio of synovial mesenchymal stem cels:chondrocytes=1:2) and group D (scaffold material without cels), were cultured on chitosan/type I colagen composite scaffolds and transplanted into the subcutaneous layer of rats folowed by morphological observation and immunohistochemical staining at 4 and 8 weeks. . <br> RESULTS AND CONCLUSION:After 4 and 8 weeks, the discoid-like scaffold was visible. The immunohistochemical staining of type II colagen and the toluidine blue staining of aggrecan were significantly positive in groups A and C. These results show that the co-culture of synovial mesenchymal stem cels and chondrocytes on the scaffold in vivocan form cartilage-like tissues.关键词
组织构建/软骨组织工程/滑膜间充质干细胞/软骨细胞/共培养技术/Ⅱ型胶原/蛋白聚糖/国家自然科学基金Key words
Synovial Membrane/Mesenchymal Stem Cels/Chondrocytes/Coculture Techniques/Colagen Type II/Tissue Engineering分类
医药卫生引用本文复制引用
杨萌,胡露露,王玥,胡鑫,林兆全,邵博,龚忠诚,宁晓婷,刘慧,克热木•阿巴斯,凌彬,尹小朋,王冰..三维环境下软骨细胞诱导滑膜间充质干细胞向软骨样细胞的分化[J].中国组织工程研究,2016,20(11):1544-1550,7.基金项目
国家自然科学基金项目(31260229)；新疆维吾尔自治区青年科技创新人才培养工程项目(2014721046)Funding:the National Natural Science Foundation of China, No.31260229 （31260229）
the Project for Training Youth Talents of Scientific Innovation in Xinjiang Uygur Autonomous Region, No.2014721046 （）
