miR-25对P19细胞向心肌细胞分化的影响及机制OA北大核心CSTPCD
Effect of miR-25 on the differentiation of P19 cells into cardiomyocytes and its mechanism
背景：既往研究发现，miR-25在向心肌分化的P19细胞中的表达水平显著低于其在未分化的P19细胞中的表达水平，但是其对心脏发育的影响及其机制尚不明确。<br> 目的：探索miR-25对P19细胞向心肌细胞分化的影响及其机制。<br> 方法：体外培养P19细胞并向心肌细胞诱导分化。通过实时反转录PCR检测miR-25在向心肌分化的和未分化的P19细胞中的表达水平。通过脂质体转染构建miR-25过表达的P19细胞，观察m…查看全部>>
BACKGROUND:Previous studies have found that the expression level of miR-25 in differentiated P19 cels is significantly lower than that in undifferentiated P19 cels. However, the effect of miR-25 on cardiomyogenesis and the relevant mechanism remain unclear. <br> OBJECTIVE: To explore the effect and mechanism of miR-25 on the differentiation of P19 cels into cardiomyocytes. <br> METHODS:P19 cels were cultured and differentiated into cardiomyocytes…查看全部>>
李连冲
南阳医学高等专科学校第一附属医院心血管外科，河南省南阳市 473000
医药卫生
组织构建组织工程心肌细胞miR-25P19细胞分化先天性心脏病Pax3
Myocytes, CardiacMicroRNAsCel Differentiation
《中国组织工程研究》 2016 (11)
1584-1590,7
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