中国组织工程研究2016，Vol.20Issue(11)：1591-1596,6.DOI:10.3969/j.issn.2095-4344.2016.11.011
巨噬细胞中脂多糖诱导微小RNA-155表达与地塞米松的抑制
Dexamethasone inhibits the expression of microRNA-155 in macrophages induced by lipopolysaccharide
王中华 1王首红 1吴岩 1李宙 1廖小龙 1覃铁和1
作者信息
- 1. 广东省人民医院 广东省医学科学院，广东省老年医学研究所重症医学科，广东省广州市 510080
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摘要
Abstract
BACKGROUND:It is unclear about dexamethasone effect on the regulation of microRNA-155 expression in macrophages. <br> OBJCTIVE:To explore whether dexamethasone can regulate the expression of microRNA-155 in macrophages. <br> METHODS:(1) Lipopolysaccharide stimulation of mouse macrophages: mouse macrophage cel lines, Raw264.7 cels, were culturedin vitro and stimulated by lipopolysaccharide. Cultured cels were colected at 0, 0.5, 2, 6 hours after culture to detect the dynamical expression of microRNA-155. (2) Dexamethasone intervention for macrophages: Macrophages were divided into four groups: control group treated with phosphate buffer; lipopolysaccharide group stimulated by lipopolysaccharide; combined group given intervention with dexamethasone and lipopolysaccharide; dexamethasone group cultured with dexamethasone. At 6 hours after culture, cel supernatant was colected to detect the expression of tumor necrosis factor α and interleukin-6 using ELISA method. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-155 in the Raw264.7 macrophages. <br> RESULTS AND CONCLUSION:Lipopolysaccharide significantly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 after 6 hours of culture (P < 0.05). Combined use of dexamethasone and lipopolysaccharide slightly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 (P< 0.05). Dexamethasone alone had no influence on the expression of tumor necrosis factor α and interleukin-6, but significantly decreased the expression of microRNA-155 (P < 0.05). These findings indicate that dexamethasone can inhibit the expression of microRNA-155 in the macrophages induced by lipopolysaccharide.关键词
组织构建/组织工程/微小RNA-155/巨噬细胞/地塞米松/脂多糖/炎症Key words
MicroRNAs/Macrophages/Lipopolysaccharides分类
医药卫生引用本文复制引用
王中华,王首红,吴岩,李宙,廖小龙,覃铁和..巨噬细胞中脂多糖诱导微小RNA-155表达与地塞米松的抑制[J].中国组织工程研究,2016,20(11):1591-1596,6.
