中国组织工程研究2016，Vol.20Issue(12)：1745-1752,8.DOI:10.3969/j.issn.2095-4344.2016.12.011
应用不同物理加强条件的Ⅰ型胶原支架的大白鼠动物模型与组织学分析
Histological evaluation of type I collagen scaffolds preparde under different dehydrothermal cross-linking conditions in a rat model
张聪 1张艳勤 1Mark Spilker 1Myron Spector 2李登云 3鲁玉梅 1许和平1
作者信息
- 1. 北京银河巴马生物技术股份有限公司，北京市 101111
- 2. 美国骨骼肌肉移植基金会，美国 02101
- 3. 美国哈佛医学院，美国波士顿 02138
- 折叠
摘要
Abstract
BACKGROUND:In previous studies, the dehydrothermal cross-linking method was modified by the authors to improve the degradation property of colagen scaffolds. The cross-linking time was increased from 24 to 48 hours, and the cross-linking temperature increased from 105 to 115℃. OBJECTIVE:To verify the anti-degradation ability of colagen scaffolds prepared using the modified dehydrothermal cross-linking method and to obtain the optimal efficacy of the scaffolds on damaged tissue repair and regeneration. METHODS: Highly-purified type I colagen scaffolds with native triple helix structure were prepared and subjected to three different dehydrothermal cross-linking conditions: 105℃ for 24 hours, 105℃ for 48 hours and 115℃ for 24 hours. Material samples, 1 cm×1 cm, were implanted subcutaneously into the rat dorsum. The specimens were harvested at 3 days, 14 days and 42 postoperative days folowed by fixation and histological analysis using hematoxylin-eosin staining. RESULTS AND CONCLUSION:No untoward foreign body and immunological reactions were observed in any groups. In the group of 105℃ for 48 hours, the scaffold retention and degree of pore openness were better than the other two groups at 14 days after scaffold implantation (P < 0.05). These findings indirectly suggest that the anti-degradation ability of colagen scaffolds can be strengthened under certain dehydrothermal cross-linking conditions: the cross-linking time is increased from 24 to 48 hours.关键词
生物材料/材料相容性/胶原/支架/高温脱水/交联/降解/组织再生分类
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张聪,张艳勤,Mark Spilker,Myron Spector,李登云,鲁玉梅,许和平..应用不同物理加强条件的Ⅰ型胶原支架的大白鼠动物模型与组织学分析[J].中国组织工程研究,2016,20(12):1745-1752,8.
