中国组织工程研究2016，Vol.20Issue(14)：2040-2045,6.DOI:10.3969/j.issn.2095-4344.2016.14.009
胃癌微环境对大鼠骨髓间充质干细胞形态、生长及CD34、CD44表达的影响
Morphology and proliferation of bone marrow mesenchymal stem cells and expressions of CD34 and CD44 under stomach cancer microenvironment
摘要
Abstract
BACKGROUND:Gastric cancer mesenchyal stem cel s from clinical stomach cancer specimens and tumorigenic tissues in nude mice are similar to the bone marrow mesenchymal stem cel s in biological characteristics, which have been proved to be an important component of tumor microenvironment to promote tumor growth. It is speculated that biological characteristic of bone marrow mesenchymal stem cel s may change in stomach cancer microenvironment. OBJECTIVE:To observe the effect of stomach cancer microenvironment on morphology and proliferation of bone marrow mesenchymal stem cel s and expressions of CD34 and CD44. METHODS:Rat bone marrow mesenchymal stem cel s were cultured alone as control group. In the test group, rat bone marrow mesenchymal stem cel s were co-cultured with human stomach cancer BGC-823 cel s using Transwel chamber assay to establish the stomach cancer microenvironment. Then, cel morphology, proliferation, cel cycle and CD34, CD44 expressions were observed and detected using inverted phase contrast microscope, MTT assay, and flow cytometry, respectively. RESULTS AND CONCLUSION:In the test group, bone marrow mesenchymal stem cel s were similar to human stomach cancer cel s BGC-823 that arranged disorderly and irregularly, were interconnected loosely, became thinner and longer, and grew in clusters with smal er nuclei. The cel proportion in G 1 phase significantly decreased, but that in S and G 2/M phases significantly increased (P<0.01, P<0.05). The positive rate of CD44 significantly declined, and the CD34 expression significantly raised (P<0.01). In conclusion, stomach cancer microenvironment by non-contact co-culture with BCG-823 cel s has an obvious effect on the morphology, proliferation and surface antigens expressions of bone marrow mesenchymal stem cel s that wil tend to be malignant gastric cancer cel s.关键词
干细胞/骨髓干细胞/胃癌微环境/骨髓间充质干细胞/细胞增殖/CD34/CD44/国家自然科学基金分类
医药卫生引用本文复制引用
吴高峰,刘喜平,杨柏林,李沛清,明海霞,张炜..胃癌微环境对大鼠骨髓间充质干细胞形态、生长及CD34、CD44表达的影响[J].中国组织工程研究,2016,20(14):2040-2045,6.基金项目
国家自然科学基金项目(81260525) （81260525）
