中国组织工程研究2016，Vol.20Issue(14)：2066-2072,7.DOI:10.3969/j.issn.2095-4344.2016.14.013
CD133+细胞联合人脐血干细胞在心力衰竭小鼠中的作用
CD133+cells in combination with human umbilical cord stem cells in mouse heart failure
摘要
Abstract
BACKGROUND:Currently, conventional treatment methods for heart failure are al ineffective. OBJECTIVE:To explore the combined effects of human umbilical cord stem cel s and CD133+cel s in mice with heart failure, providing a new insight into the treatment of heart failure. METHODS:Ful-term newborn umbilical cord from vaginal delivery was col ected to isolate CD133+cel s and human umbilical cord stem cel s using lymphocyte separation medium method. Twenty Balb/C nude mice were randomly subjected to mononuclear cel injection (mononuclear cel group) or injection of CD133+cel s combined with human umbilical cord stem cel s (combined group) via the tail vein after establishing heart failure models. RESULTS AND CONCLUSION:Fourteen days after injection, the body weight and liver, heart and lung mass of mice were significantly larger in the combined group than the mononuclear cel group (P<0.05). After 30 days, myocardial cel s arranged regularly in the combined group, but disorderly in the mononuclear cel group;compared with the mononuclear cel group, the average area of myocardial col agen fibers was significantly decreased in the combined group (P<0.05), and the level of serum matrix metal oproteinase-9 was also significantly lower in the combined group (P<0.05). Masson staining showed that blue-stained col agen fibers in the combined group were less but arranged neatly;however, in the mononuclear cel group, the number of col agen fibers that arranged irregularly was increased to different extents. To conclude, the combined use of CD133+cel s and human umbilical cord stem cel s has desired outcomes in the treatment of heart failure in mice, indicating a higher clinical value.关键词
干细胞/移植/人脐血干细胞/CD133+细胞/心力衰竭/协同作用/治疗效果/单个核细胞/血清基质金属蛋白酶9/Masson染色/国家自然科学基金分类
医药卫生引用本文复制引用
后梅,张红星,叶青,张勇..CD133+细胞联合人脐血干细胞在心力衰竭小鼠中的作用[J].中国组织工程研究,2016,20(14):2066-2072,7.基金项目
国家自然科学基金项目(81560462) （81560462）
