中国组织工程研究2016，Vol.20Issue(15)：2156-2162,7.DOI:10.3969/j.issn.2095-4344.2016.15.004
膝关节软骨早期缺损修复中的基质金属蛋白酶3抑制剂Ⅰ
Matrix metalloproteinase-3 inhibitor I accelerates the early-stage repair of full-thickness articular cartilage defects in the knee of rats
摘要
Abstract
BACKGROUND:The biomechanical properties of naturaly regenerated damaged articular cartilage that belongs to the fibrovascular tissue are far worse than those of the normal cartilage so that they cannot meet the requirements for joint function, leading to traumatic arthritis and loss of joint function. <br> OBJECTIVE:To evaluate the effects of matrix metaloproteinase-3 (MMP-3) inhibitor I with different concentrations on the early-stage repair of ful-thickness articular cartilage defects in the knee of rats. <br> METHODS: Twenty-four Sprague Dawley rats were randomized into control, defect (DEF), and defect combined with low-(D+L) and high-dose inhibitor (D+H) groups (n=6 for each group), respectively. Full-thickness articular cartilage defects followed by intraarticular injection of low- and high-dose MMP-3 inhibitor I for 4 weeks was administered in the later two groups. Serum MMP-3 was detected using ELISA method before and after experiment, respectively. Femoral trochleas were collected to observe characteristics of repaired tissue by gross appearance scoring and O’Driscoll histological scoring with Safranine O-Fast Green staining, and to measure type II colagen by immunohistochemistry after experiment. <br> RESULTS AND CONCLUSION:Rats in the D+H group had obvious repair similarly to hyaline articular cartilage, while creamy white cartilage tissue and fibrous tissue repair were observed in D+L group and in DEF group. D+H group obtained the best repair results according to gross appearance scoring and O’Driscol histological scoring and the highest content of type II colagen (P< 0.05). MMP-3 concentration and the difference value before and after experiment were gradualy decreased in DEF, D+L, D+H, and control groups in sequence(P< 0.05). These findings demonstrate that MMP-3 inhibitor I accelerates the early-stage repair of ful-thickness articular cartilage defects in the knee of rats.关键词
组织构建/软骨组织工程/基质金属蛋白酶3抑制剂Ⅰ/关节软骨缺损/早期修复/基质金属蛋白酶3,组织学观察/免疫组织化学/Ⅱ型胶原分类
医药卫生引用本文复制引用
董福,宋锦旗,姜楠,陆春..膝关节软骨早期缺损修复中的基质金属蛋白酶3抑制剂Ⅰ[J].中国组织工程研究,2016,20(15):2156-2162,7.基金项目
北海市科学研究与技术开发计划项目(201405003)；深圳市卫生计生系统博士创新项目资助课题(201505028)@@@@the Scientific Research and Technology Development Program of Beihai City, China, No.201405003 （201405003）
the Medical Doctoral Innovation Project Funding of Shenzhen Health Development Planning Commission, No.201505028 （）
