中国组织工程研究2016，Vol.20Issue(15)：2184-2189,6.DOI:10.3969/j.issn.2095-4344.2016.15.008
甘草黄酮对运动大鼠骨骼肌Perilipin mRNA及蛋白表达的影响
Effects of Glycyrrhiza flavonoids on expression of perilipin mRNA and protein in skeletal muscle of rats after exhaustive exercise
摘要
Abstract
BACKGROUND:Glycyrrhiza flavonoids can protect muscle tissues against oxidative and inflammatory injuries. <br> OBJECTIVE:To explore the effects of Glycyrrhiza flavonoids on energy storing and release of adipose tissue by studying expressions of perilipin mRNA and protein in skeletal muscle tissues of rats after exhaustive exercise. <br> METHODS:Fifty male healthy Sprague-Dawley rats were used and equaly randomized into five groups: quiet control, exercise alone (intragastric administration with saline), exercise combined with low-, moderate-, high-dose Glycyrrhiza flavonoids (intragastric administration with Glycyrrhiza flavonoids) groups, respectively. Perilipin mRNA and protein expressions in skeletal muscle tissues containing gastrocnemius muscle and musculi soleus were determined at 6 weeks after exhaustive exercise. <br> RESULTS AND CONCLUSION:Expression of perilipin mRNA in rat gastrocnemius muscle in quiet control group was significantly decreased compared with exercise alone and al combined intervention groups (P < 0.05 orP < 0.01). Protein expression of perilipin in exercise combined with moderate- or high-dose glycyrrhiza flavonoids group was significantly increased compared with quiet control group (P < 0.05). Expression of perilipin mRNA in rat musculi soleus was significantly decreased compared with exercise combined with moderate-dose glycyrrhiza flavonoids group (P < 0.05). These findings confirm that Glycyrrhiza flavonoids are benefit for improvement of aerobic metabolism capacity of gastrocnemius muscle through regulating lipolysis pathway.关键词
组织构建/组织工程/甘草黄酮/运动/大鼠/围脂滴蛋白/腓肠肌/国家自然科学基金分类
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王成科,莫伟彬,潘昌红,张蒙,黄东..甘草黄酮对运动大鼠骨骼肌Perilipin mRNA及蛋白表达的影响[J].中国组织工程研究,2016,20(15):2184-2189,6.基金项目
国家自然科学基金项目(31460232，21562006)；广西自然科学资金面上项目(2015GXNSFAA139186)；“药用资源化学与药物分子工程教育部重点实验室”开放课题(CMEMR2012-B04).@@@@the National Natural Science Foundation of China, No.31460232,21562006 （31460232，21562006）
the Natural Science Foundation of Guangxi, No.2015GXNSFAA139186 （）
State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China Open Projects Fund, No. CMEMR2012-B04 （）
