中国组织工程研究2016，Vol.20Issue(15)：2218-2224,7.DOI:10.3969/j.issn.2095-4344.2016.15.013
FABP-5基因沉默对宫颈癌SiHa细胞生物学特性的影响
Effects of FABP-5 gene silencing on the biological characteristics of the cervical carcinoma cell line SiHa
陈逢振 1尹利荣1
作者信息
- 1. 天津医科大学第二医院妇科，天津市 120000
- 折叠
摘要
Abstract
BACKGROUND:Cancer related genes and pathways play a critical role in formation and development of cancer. <br> OBJECTIVE: To investigate the silencing epidermal fatty acid binding protein 5 (FABP-5) by siRNA interference on biological characteristics of the cervical carcinoma cel line SiHa. <br> METHODS:Design and synthesis of siRNA interference sequence used to transiently transfect SiHa cels was performed according to FABP-5 mRNA coding sequence. mRNA and protein expressions of FABP-5 were detected by RT-PCR and western blot assay, respectively. Meanwhile, cel cycle, proliferation, invasion and apoptosis were detected by flow cytometry, cel counting bit-8 assay, Boyden chamber assay, and TUNEL staining, respectively. <br> RESULTS AND CONCLUSION: FABP-5 mRNA and protein expressions, cel growth speed, cel number at S phase (cel cycle was arrested at the G0/G1 phase) and cel invasion were significantly decreased, while the cel apoptosis was significantly increased in FABP-5 siRNA group compared with the negative control and blank control groups. Our findings indicate that the specific silencing FABP-5 gene expression by siRNA interference can inhibit SiHa cervical cel growth and proliferation, but accelerate cel apoptosis. Subject headings: RNA, Smal Interfering; Fatty Acid-Binding Proteins; Uterine Cervical Neoplasms; Cel Proliferation; Apoptosis关键词
组织构建/组织工程/宫颈癌/FABP-5/siRNA干扰/SiHa细胞/细胞周期/细胞增殖/细胞侵袭/细胞凋亡分类
医药卫生引用本文复制引用
陈逢振,尹利荣..FABP-5基因沉默对宫颈癌SiHa细胞生物学特性的影响[J].中国组织工程研究,2016,20(15):2218-2224,7.
