中国组织工程研究2016，Vol.20Issue(17)：24529-24534,6.DOI:10.3969/j.issn.2095-4344.2016.17.019
成骨分化与修复调控转录因子Runx2在股骨头坏死组织中的表达：非随机平行对照临床观察试验方案
Osteogenesis-related factor Runx2 expression in necrotic femoral head tissue：study protocol for a non-randomized, parallel-controlled trial
摘要
Abstract
BACKGROUND:Osteonecrosis of the femoral head (ONFH) results from an interruption of the blood supply to the femoral head or injury-caused death of chondrocytes and bone marrow components. Many osteogenesis-related factors, such as Runt-related transcription factor 2 (Runx2), bone morphogenetic protein (BMP), osteoprotegerin and osteocalcin, are involved in the subsequent repair process. The Runx2 gene has been shown to control osteoblast differentiation and formation by upregulating the transcription of various mineralization-related protein genes, alowing osteoblast precursors differentiation into osteoblasts, and playing a key role in bone repair. <br> METHODS/DESIGN:This retrospective, single-center, non-randomized, paralel-controled trial was performed at Shengjing Hospital of China Medical University, China. Fifteen patients with ONFH and 15 patients with osteoarthritis undergoing total hip replacement were included. Primary measure was Runx2messenger RNA (mRNA) expression in femoral head and neck tissue, and secondary measures included BMP-2, BMP-7 and osteoprotegerin mRNA expression, as wel as osteocalcin immunoreactivity. This trial was registered at ClinicalTrials.gov (identifier: NCT02735538) and acquired written approval from the Ethics Committee of Shengjing Hospital of China Medical University (approval No. 2016PS222K). Written informed consent was obtained from each included patient. <br> DISCUSSION:This study involved in the detection of mRNA expression of several bone repair-related genes, including Runx2, in the femoral head and neck of patients with ONFH was performed between 2010 and 2011. This observational study wil help clarify the role and possible clinical significance of Runx2 in femoral head necrosis, bone repair and reconstruction.关键词
骨科植入物/人工假体/股骨头坏死/骨关节炎/Runx2/骨形态发生蛋白/骨保护素/骨钙素/非随机对照试验分类
医药卫生引用本文复制引用
杨礼庆,董成建,朱姝..成骨分化与修复调控转录因子Runx2在股骨头坏死组织中的表达：非随机平行对照临床观察试验方案[J].中国组织工程研究,2016,20(17):24529-24534,6.基金项目
辽宁省科学技术计划项目(2009225010-15)@@@@the High-Tech Research and Development Program of Liaoning Province of China, No.2009225010-15 （2009225010-15）
