中国组织工程研究2016，Vol.20Issue(18)：2717-2724,8.DOI:10.3969/j.issn.2095-4344.2016.18.020
猫视乳头三维模型重建及有限元分析
Three-dimensional reconstruction and finite element analysis of the optic nerve head of a cat
摘要
Abstract
BACKGROUND: Glaucoma is a kind of eye disease that can cause irreversible blindness which is characterized by visual field loss. Clinical research shows that the optic nerve head has changed before the visual field loss. The morphological changes of the optic nerve head have become the key to determine the early diagnosis of glaucoma and disease development. So it has important significance for us to study the morphological changes of the tissues of optic nerve head under the high intraocular pressure. <br> OBJECTIVE: To establish three-dimensional finite element model of optic nerve head which includes choroid, retina and lamina cribrosa, and analyze the thickness of the optic nerve head under the acute high intraocular pressure. <br> METHODS: (1) We chose healthy cats without refractive media and other eye diseases. The tomographic sequence images of the optic nerve head of a cat were obtained with the intraocular pressure of the normal value by using enhanced depth imaging spectral-domain optical coherence tomography. (2) The three-dimensional model of the retina, choroid and lamina cribrosa was obtained. Three-dimensional model of optic nerve head was established by assembling the model of the retina, the choroid and the lamina cribrosa. The thickness of the retina, choroid and lamina cribrosa was analyzed under different intraocular pressures by using the method of finite element analysis. (3) Animal model of acute ocular hypertension was established by methods of anterior chamber perfusion. The tomographic sequence images of the optic nerve head of a cat were obtained with different intraocular pressures by using enhanced depth imaging spectral-domain optical coherence tomography. Then, we measured the thickness changes of the choroid, retina and lamina cribrosa under different intraocular pressures, and compared with the results of finite element analysis. <br> RESULTS AND CONCLUSION: The thickness of the choroid, retina and lamina cribrosa trended to be thin, and the cup dish ratio of optic nerve head increased gradual y with the increased intraocular pressures. Regarding to the thickness changes of the choroid, retina and lamina cribrosa, the trend of experimental measurement results was consistent with finite element calculation results. Hence, it is feasible to analyze morphological changes of every fundus tissue under high intraocular pressure using the tomographic sequence images obtained by optical coherence tomography. We can predict the morphological changes of the optic nerve head tissue by finite element analysis, which has certain guiding significance in determining the progression of glaucoma.关键词
实验动物/细胞损伤与修复模型/有限元分析/视网膜/脉络膜/筛板/急性高眼压/三维重建/青光眼/杯盘比/国家自然科学基金分类
医药卫生引用本文复制引用
高伟,杨昱童,张诗瑶,张昆亚,刘志成,钱秀清..猫视乳头三维模型重建及有限元分析[J].中国组织工程研究,2016,20(18):2717-2724,8.基金项目
国家自然科学基金(11102123，31570952)；北京市自然科学基金(7142024)@@@@the National Natural Science Foundation of China, No.11102123, No.31570952 （11102123，31570952）
the Natural Science Foundation of Beijing, No.7142024 （）
